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Publication
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| Title of Article |
Assessment of caprine corpora lutea growth, progesterone concentration and eNOS expression: Effect of a compensatory gain model |
| Date of Acceptance |
25 February 2016 |
| Journal |
| Title of Journal |
Domestic Animal Endocrinology |
| Standard |
ISI |
| Institute of Journal |
Elsevier |
| ISBN/ISSN |
0739-7240 |
| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2016 |
| Page |
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| Abstract |
The experiment was conducted to evaluate corpus luteum (CL) growth, progesterone (P4) concentration and endothelial nitric oxide
synthase (eNOS) expression in nutrient stair-step fed goats. Female
goats (n = 32) that exhibited at least two, normal, consecutive estrous
cycles were randomly assigned to either the control or stair-step fed
group. In the control group, goats were fed ad libitum (100% of nutrient
requirement for goats). The goats in the stair-step group were fed 70% of
the control consumption for the first 42 days and 130% for the later 42
days during four consecutive estrous cycles (84 days). Blood and luteal
samples were collected on days 3, 8, 13, 18 of the estrous cycle to
determine concentration of glucose, insulin, P4, luteal growth, and eNOS
expression. Luteal growth was determined using fresh CL weight, DNA
content, DNA and protein concentrations, and cell proliferation (labeling
index of Ki67). During realimentation phase at 4 h, glucose and insulin
concentrations were greater (P < 0.05) in stair-step fed goat than
control goats. Fresh CL weight, DNA content, protein concentrations and
labeling index of Ki67 on day 8 of the estrous cycle in the stair-step
group were greater (P < 0.05) than that of the control group. Protein
for eNOS was located in the capillaries of CL throughout of the estrous
cycle in both groups. Greater serum P4 concentrations and eNOS protein
(P < 0.05) were observed in the stair-step fed goats on day 3 (1.83 ng/mL
and 6.79%) compared to the control goats (0.98 ng/mL and 6.02%), and on
day 8 (5.15 ng/mL and 7.88%) compared to the control goats (4.54 ng/mL
and 7.07%). These data demonstrate that luteal growth, progesterone
concentration, and eNOS protein were partially affected by nutrient
compensatory gain in goats. |
| Keyword |
luteal growth, progesterone, eNOS protein, restriction, realimentation, goats |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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