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Journal Publication
Research Title Antimicrobial and antiadhesive activity of the biosurfactant from Bacillus spp. against Burkholderia pseudomallei and nosocomial pathogens  
Date of Distribution 15 January 2016 
     Title of the Conference The National and International Graduate Research Conference 2016 
     Organiser Graduate School, Khon Kaen University, Thailand and Universitas Muhammadiyah Yogyakarta, Indonesia 
     Conference Place Pote Sarasin Building, Khon Kaen University 
     Conference Date 15 January 2016 
     To 15 January 2016 
Proceeding Paper
     Page 239 
     Editors/edition/publisher Graduate School, Khon Kaen University 
     Abstract The aims of this study to determine anti-microbial and anti-adhesive activity of the biosurfactant (BS) from Bacillus spp. against B. pseudomallei and pathogens that cause of hospital-acquired infection also known as nosocomial pathogens. Four crude BS samples were extracted from 2 isolates of B. subtilis and 2 isolates of B. amyloliquefaciens and screened surfactant activities by oil spreading and emulsification assay. The result showed that crude BS KKU14 from B. amyloliquefaciens have more surfactant activities than other BS samples. Then, crude BS KKU14 was determined antimicrobial activity on 96-well plate by co-culture between pathogens and BS compare with growth control. The result showed that crude BS KKU14 strong inhibit the growth of 3 clinical isolates of B. pseudomallei in range of 19-24%, 23-27%, and 30-57% at 5, 10 and 20 mg/ml, respectively. Crude BS at 50, 100 and 150 mg/ml could inhibit 2 clinical B. pseudomallei in range of 69-76%, 82-83%, 78-85%, respectively while could inhibit 4 nosocomial pathogens only at 150 mg/ml in range of 59-78%. Moreover, the crude BS KKU14 has anti-adhesive activity against 4 nosocomial pathogens on intravenous (IV) tube when compare with untreated tube. Therefore, BS may be used as a cleansing agent for washing the equipments in laboratory or a coating agent on medical devices for prevention the pathogens contamination particular implanted medical for long term.  
565070010-1 Mr. PONTAPAN POLYIAM [Main Author]
Medicine Master's Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis false 
Presentation awarding false 
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