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Publication
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| Title of Article |
SXT element, class 1 integron and multidrug-resistance genes of Vibrio cholerae isolated from clinical and environmental sources in northeast Thailand |
| Date of Acceptance |
15 August 2016 |
| Journal |
| Title of Journal |
The Southeast Asian journal of tropical medicine and public health |
| Standard |
ISI |
| Institute of Journal |
The SEAMEO Regional Tropical Medicine and Public Health Network (SEAMEO TROPMED Network). |
| ISBN/ISSN |
0125-1562 |
| Volume |
47 |
| Issue |
5 |
| Month |
September |
| Year of Publication |
2016 |
| Page |
957-966 |
| Abstract |
Emergence of multiple drug resistance in Vibrio cholerae has been
increasing around the world including Northeast Thailand. In this study, 92
isolates of V. cholerae (50 O1 and 42 non-O1/non-O139 isolates) from clinical
and environmental sources in Northeast Thailand were randomly selected
and investigated for the presence of SXT element, class 1 integron and antimicrobial
resistance genes. Genotypic-phenotypic concordance of antimicrobial
resistance was also determined. Using PCR-based assays, 79% of V. cholerae
isolates were positive for SXT element, whereas only 1% was positive for
class 1 integron. SXT element harbored antimicrobial resistance genes, dfrA1
or dfr18, floR, strB, sul2, and tetA. Overall phenotypic-genotypic concordance
of antimicrobial resistance was 78%, with highest and lowest value being for
trimethoprim (83%) and chloramphenicol (70%), respectively. Ninety-two
percent of V. cholerae O1 strains isolated from clinical sources harbored both
dfrA1 (O1-specific trimethoprim resistance gene) and dfr18 (non-O1-specific trimethoprim
resistance gene), whereas only 5% of V. cholerae non-O1/non-O139
strains harbored both genes. All V. cholerae O1 isolated from environmental
source harbored dfr18 but 48% of V. cholerae non-O1/non-O139 harbored dfrA1.
This study indicates that SXT element was the main contributor to the circulation
of multiple-drug resistance determinants in V. cholerae strains in Northeast
Thailand and that genetic exchange of SXT element can occur in both V. cholerae
O1 and non-O1/non-O139 strains from clinical and environmental sources. |
| Keyword |
Vibrio cholerae, antimicrobial resistance, class 1 integron, clinical source, environmental source, SXT element |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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