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Publication
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| Title of Article |
Identification of peanut seed prolamins with an
antifungal role by 2D-GE and drought treatment |
| Date of Acceptance |
1 May 2015 |
| Journal |
| Title of Journal |
Bioscience, Biotechnology, and Biochemistry |
| Standard |
ISI |
| Institute of Journal |
Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2015 |
| Page |
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| Abstract |
This work revealed peanut seed prolamins likely
displaying a defensive role besides the known nitrogen
storage. Drought stress and proteomic
approaches were used in varieties of peanuts to
explore the prolamin member in association with a
test against Aspergillus flavus spore germination.
The stress effect was showed by aerial biomass, leaf
content of malondialdehyde, and seed contamination
by A. flavus. Sodium dodecyl sulfate–polyacrylamide
gel electrophoresis profiles were not informative for
the antifungal polypeptides. From two-dimensional
gel electrophoresis, the suspected polypeptides were
those with pI 5.45–5.75 and sizes of 22.0–30.5 kDa
specifically in Spanish-type peanuts. Regarding to
the drought effect in most of these peanuts, the spot
peak volume analysis deduced three novel prolaminrelated
antifungal polypeptides at pI 5.75–5.8 with
30.5, 27.5–28.5, and 22.0–22.5 kDa, which was
confirmed after isoelectric purification at pH 5.60.
The data could not yet conclude their correlation
with resistance to drought and to seed infection by
A. flavus. |
| Keyword |
2D-GE maps; antifungus; Aspergillus flavus; peanut seed prolamins |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Attach file |
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| Citation |
0
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Journal Publication
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