2012 ©
Journal Publication
Title of Article Identification of peanut seed prolamins with an antifungal role by 2D-GE and drought treatment 
Date of Acceptance 1 May 2015 
     Title of Journal Bioscience, Biotechnology, and Biochemistry 
     Standard ISI 
     Institute of Journal Japan Society for Bioscience, Biotechnology, and Agrochemistry 
     Year of Publication 2015 
     Abstract This work revealed peanut seed prolamins likely displaying a defensive role besides the known nitrogen storage. Drought stress and proteomic approaches were used in varieties of peanuts to explore the prolamin member in association with a test against Aspergillus flavus spore germination. The stress effect was showed by aerial biomass, leaf content of malondialdehyde, and seed contamination by A. flavus. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis profiles were not informative for the antifungal polypeptides. From two-dimensional gel electrophoresis, the suspected polypeptides were those with pI 5.45–5.75 and sizes of 22.0–30.5 kDa specifically in Spanish-type peanuts. Regarding to the drought effect in most of these peanuts, the spot peak volume analysis deduced three novel prolaminrelated antifungal polypeptides at pI 5.75–5.8 with 30.5, 27.5–28.5, and 22.0–22.5 kDa, which was confirmed after isoelectric purification at pH 5.60. The data could not yet conclude their correlation with resistance to drought and to seed infection by A. flavus. 
     Keyword 2D-GE maps; antifungus; Aspergillus flavus; peanut seed prolamins 
537020026-7 Miss WARALUK SENAKOON [Main Author]
Science Doctoral Degree

Reviewing Status มีผู้ประเมินอิสระ 
Status ได้รับการตอบรับให้ตีพิมพ์ 
Level of Publication นานาชาติ 
citation true 
Part of thesis true 
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