2012 ©
Journal Publication
Research Title Opisthorchis viverrini infection exacerbates the severity of diabetic liver injury and non-alcoholic fatty liver disease 
Date of Distribution 14 November 2016 
     Title of the Conference ASTMH 65th Annual Meeting 
     Organiser American Society of Tropical Medicine and Hygiene 
     Conference Place Atlanta Marriott Marquis and Hilton Atlanta 
     Province/State Atlanta, Georgia 
     Conference Date 13 November 2016 
     To 17 November 2016 
Proceeding Paper
     Volume 65 
     Page 178 
     Abstract The highest co-prevalence between infection with Opisthorchis viverrini and diabetes mellitus (DM) is found in the northeastern Thailand. In ammatory responses that lead to hepatobiliary disease is seen during opisthorchiasis. Moreover, non-alcoholic fatty liver disease (NAFLD) and diabetic liver injury caused by DM lead to the majority burden of liver disease at large. However, the association among opisthorchiasis, DM and hepatobiliary disease have not been clari ed. The aim of these studies was to investigate the effect of O. viverrini infection on the development and progression of DM, NAFLD and diabetic liver injury in models using hamsters and cell lines. An experiment was carried out on four groups of hamsters: (1) normal control (NC), (2) chronic opisthorchiasis (OV), (3) NC and HFD fed with 10% fructose in drinking water for short-term (one month) and long-term (four months) (HF), and (4) O. viverrini infection at 4 months and followed by HFD fed with 10% fructose in drinking water for one and four month(s) (OVHF). Hamsters were euthanized at designated time points at ve and eight months following infection. The fasting blood glucose levels of all experimental groups did not differ signi cantly. Intriguingly, the homeostatic model assessment of insulin resistance (HOMA-IR) in short-term HF treatment of group 3 was higher than other groups at the same time point. However, the HOMA-IR of long-term HF treatment of group 3 was not different from OVHF group but higher than OV group at the same time point. Histological features of hamster livers revealed that the highest amount of lipid droplet was found in short-term HF treatment of OVHF group. In addition, we observed that excretory/secretory products of O. viverrini suppressed the growth of HepG2 cell line by dose-dependent manner when monitored under real- time monitoring system. These ndings indicate the effect of O. viverrini on the improvement of insulin sensitivity. Infection with O. viverrini might inhibit regeneration of hepatocytes and increase the severity of diabetic liver injury, and hence chronic opisthorchiasis might represent a risk factor for NAFLD. 
557070026-9 Mr. APISIT CHAIDEE [Main Author]
Medicine Doctoral Degree
587070027-0 Miss ORNUMA HAONON
Medicine Doctoral Degree
Medicine Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding false 
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