| Research Title |
EBV-encoded small RNAs (EBERs)-containing exosomes induce an immunosuppressive protein IDO in epithelial cells via IL-6 pathway |
| Date of Distribution |
25 October 2017 |
| Conference |
| Title of the Conference |
The 65th Annual Meeting of Japanese Society for virology |
| Organiser |
Japanese Society for virology |
| Conference Place |
Osaka |
| Province/State |
Japan |
| Conference Date |
24 October 2017 |
| To |
26 October 2017 |
| Proceeding Paper |
| Volume |
65 |
| Issue |
1 |
| Page |
287 |
| Editors/edition/publisher |
|
| Abstract |
[Background]
Our previous study has shown that some macrophages in the tumor stromal of oral squamous cell carcinoma (OSCC) tissue expressed the immunosuppressive protein indoleamine 2, 3-dioxygenase (IDO) more strongly than did tumor cells. In addition, IDO can be secreted from EBV infected B cells and inhibit NK cell cytotoxicity. EBV components in infected cells as well as both of Epstein-Barr Virus (EBV)-encoded small RNAs (EBER); EBER-1 and EBER-2 can be also carried via exosomes which modulate biological changes in neighboring cells.
[Materials and Methods]
This study aimed to investigate the effect of EBERs-containing exosomes in epithelial cells on IDO induction. Two EBV positive cell lines (B95-8, P3HR1) were used for exosome isolation by using differential ultracentrifugation. qRT-PCR was used to determine the expression of EBERs, IL-6, and IDO. HEK293T cell line was used as recipient epithelial cells. EBERs-transfected HEK293T cell lines were used to confirm the effect of EBERs on IDO expression.
[Results]
Both EBER-1 and EBER-2 were detected in the culture supernatants and also in the purified exosome fractions of all EBV positive cell lines. EBV-modified exosomes induced an expression of both IL-6 and IDO in HEK293T cell after incubation with exosomes. Interestingly, the level IL-6 and IDO were further increased in a time and a dose-dependent manner in both of EBER-1 and EBER-2 transfected HEK293T cell.
[Discussion]
These results reveal that EBERs are secreted from EBV-infected cells via exosomes, internalized in the recipient epithelial cells and induces IDO expression through IL-6 pathway. This study suggest that exosomes from neighboring cells containing EBERs may promote the immunosuppressive microenvironment and induce oral cancer development.
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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