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Publication
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| Research Title |
Effects of oral feeding with probiotic bacteria on microbial population and cecum morphology of broiler chicken |
| Date of Distribution |
28 August 2018 |
| Conference |
| Title of the Conference |
2018 7th International Conference on Environment, Energy, and Biotechnology |
| Organiser |
Hong Kong Chemical, Biological & Environmental Engineering Society (HKCBEES) |
| Conference Place |
Seisa Dohto University |
| Province/State |
Kitahiroshima, Japan |
| Conference Date |
27 August 2018 |
| To |
29 August 2018 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
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| Abstract |
This study aims to determine the effects of Bacillus subtilis KKU213, C330 and lactic acid bacteria (LAB) including Pediococcus acidilactici SH8, Enterococcus feacium CA4, Lactobacillus salivarius CH24, and Enterococcus durans CH33 on microbiota in the cecum of 45-day-old male Cobb broiler chicken. Chicks were randomly divided into 13 groups, with 6 chicks per group. They were orally fed by H2O (control), 1 ml of B. subtilis KKU213 (T1), B. subtilis C330 (T2), P. acidilactici SH8 (T3), E. faecium CA4 (T4), L. salivarius CH24 (T5), and E. durans CH33 (T6), B. subtilis KKU213+LAB at days (d) 1, 3, and 5 (T7), B. subtilis C330 + LAB at d 1, 3, and 5 (T8), B. subtilis KKU213 at d 1 + LAB at d 3, and 5 (T9), B. subtilis C330 at d 1 + LAB at d 3, and 5, B. subtilis KKU213 at day 1 and 3 +LAB at d 5 (T11), and B. subtilis C330 at d 1 and 3 + LAB at d 5 (T12). Total LAB and bacteria in the cecum were counted on MRS and LB agars, respectively. LAB numbers were highest in T5 about 8.67±0.061 log CFU/g. For the total bacteria count on LB agar at 50°C for a selection of thermophillic Bacillus spp., the highest number was also observed in T6. Morphology of chicken epithelial cells in the cecum was observed by scanning electron microscopy (SEM). After feeding with probiotics, the number of villi in 45 day-old chickens was significantly increased. The number of villi in C330 (T2) was higher than KKU213 (T1) and control groups. Among the single LAB-fed groups, the number of villi present in the cecum was highest in CH24 group (T5). In addition, the chickens fed with a combination of C330+LAB strains showed a higher number of villi in cecum than KKU213+LAB strains. Therefore, feeding with a single or mixed probiotic strains was able to enhance the population of some bacteria and nutrient adsorption in broilers, possibly via an increase in their total villi. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย