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Journal Publication
Title of Article Postprandial in vitro Protease-Specific Activity of Nile Tilapia (Oreochromis niloticus, L.) Digestive Organs 
Date of Acceptance 17 October 2018 
Journal
     Title of Journal Pakistan Journal of Nutrition 
     Standard SCOPUS 
     Institute of Journal Science Alert 
     ISBN/ISSN 1680-5194 
     Volume 2019 
     Issue 18 
     Month 2
     Year of Publication 2019 
     Page 125-133 
     Abstract Background and Objective: Protease-specific activity varies postprandially. This study aimed to identify the optimum time after feeding Nile tilapia (Oreochromis niloticus, L.) to collect digestive organ samples (stomach, proximal intestine, distal intestine and liver) to obtain maximum protease-specific activity values. Materials and Methods: One hundred Nile tilapia (average weight 55.19 ± 1.78 g / fish) were acclimatized and fed two times per day for 14 days. At the beginning of the experiment, 6 fish were randomly taken from the tank before feeding (0 h), and then 6 fish were also randomly taken after feeding at 1, 3, 6, 12, 18, 24, 36 and 48 h for digestive organ collection. Homogenized organ pH was measured and extracted for a protease-specific activity assay using azocasein as a substrate. Results: The pH values of all organs differed (p < 0.05) after feeding, and the pH changes were related to chyme movement in these organs. The protease-specific activities of the stomach and proximal intestine were highest at 24 h after feeding (p < 0.05), but the protease-specific activities of the distal intestine and liver were not significantly different (p > 0.05) between time points. Conclusion: Postprandial digestive organ sample collection was recommended at 24 h to maximize protease-specific activity for further protease characterization. 
     Keyword postprandial, digestive organs, pH, protease-specific activity, Oreochromis niloticus, L. 
Author
547030023-8 Miss THATSANEE ANUKOOLPRASERT [Main Author]
Agriculture Doctoral Degree
557030013-2 Mr. KHAJORNKIAT SRINUANSOM
Agriculture Doctoral Degree
577030022-3 Mr. THANASORN RUKDONTRI
Agriculture Doctoral Degree
595030061-1 Mr. SATAPHON NONKHUKHETKHONG
Agriculture Master's Degree

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