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             Publication
Journal Publication
Research Title KT2 AND RT2 ANTIMICROBIAL PEPTIDES MODIFIED FROM SIAMESE CROCODILE LEUCROCIN I, PROMOTES ANTICANCER ACTIVITY IN HUMAN COLON CANCER HCT-116 CELLS  
Date of Distribution 29 October 2018 
Conference
     Title of the Conference The 44th Congress on Science and Technology of Thailand (STT44) 
     Organiser The Science Society of Thailand under the Patronage of His Majesty the King 
     Conference Place Bangkok International Trade & Exhibition Centre (BITEC) 
     Province/State Bangkok 
     Conference Date 29 October 2018 
     To 31 October 2018 
Proceeding Paper
     Volume 2018 
     Issue 44 
     Page 134 
     Editors/edition/publisher  
     Abstract The antimicrobial peptides are regarded as a potential solution for treating cancer cells. Therefore, the current study aimed to investigate the effect of potent cationic antimicrobial peptides, Leucrocin I modified KT2 and RT2, against the HCT-116 colon cancer cells. Using MTT assay, results demonstrated that of peptides KT2 and RT2 exhibited strong cytotoxic effect against HCT-116 human colon cancer cells with IC50 values of 90.25 and 87.84 μg/mL after 24 h treatments, respectively. However, showing no signs of cytotoxicity towards noncancerous Vero cells. Additionally, the colony count indicated that peptides (KT2 and RT2) induced a dose-dependent decrease in the ability of colony formation of HCT-116 cells. The mechanism involved in cancer cell cytotoxicity of both peptides was shown to be associated with the induction of apoptosis, as evidenced by AO/EB fluorescent staining and annexin V-FITC/PI staining flow cytometry analysis. These evidence were due to peptides consequently occurred via suppression of Bcl-2 and XIAP, and triggering p53, cytochrome c, and caspase cascade mRNA expression level. Furthermore, the peptides induced cell cycle arrest via up-regulation of cyclin-dependent kinase inhibitor p21. These collected data clearly demonstrated that peptides KT2 and RT2 offered great potential in the treatment of human colon cancer cells. 
Author
597020014-0 Mr. SURACHAI MAIJAROEN [Main Author]
Science Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding false 
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