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Publication
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| Research Title |
KT2 AND RT2 ANTIMICROBIAL PEPTIDES MODIFIED FROM SIAMESE CROCODILE LEUCROCIN I, PROMOTES ANTICANCER ACTIVITY IN HUMAN COLON CANCER HCT-116 CELLS |
| Date of Distribution |
29 October 2018 |
| Conference |
| Title of the Conference |
The 44th Congress on Science and Technology of Thailand (STT44) |
| Organiser |
The Science Society of Thailand under the Patronage of His Majesty the King |
| Conference Place |
Bangkok International Trade & Exhibition Centre (BITEC) |
| Province/State |
Bangkok |
| Conference Date |
29 October 2018 |
| To |
31 October 2018 |
| Proceeding Paper |
| Volume |
2018 |
| Issue |
44 |
| Page |
134 |
| Editors/edition/publisher |
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| Abstract |
The antimicrobial peptides are regarded as a potential solution for treating cancer cells. Therefore, the current study aimed to investigate the effect of potent cationic antimicrobial peptides, Leucrocin I modified KT2 and RT2, against the HCT-116 colon cancer cells. Using MTT assay, results demonstrated that of peptides KT2 and RT2 exhibited strong cytotoxic effect against HCT-116 human colon cancer cells with IC50 values of 90.25 and 87.84 μg/mL after 24 h treatments, respectively. However, showing no signs of cytotoxicity towards noncancerous Vero cells. Additionally, the colony count indicated that peptides (KT2 and RT2) induced a dose-dependent decrease in the ability of colony formation of HCT-116 cells. The mechanism involved in cancer cell cytotoxicity of both peptides was shown to be associated with the induction of apoptosis, as evidenced by AO/EB fluorescent staining and annexin V-FITC/PI staining flow cytometry analysis. These evidence were due to peptides consequently occurred via suppression of Bcl-2 and XIAP, and triggering p53, cytochrome c, and caspase cascade mRNA expression level. Furthermore, the peptides induced cell cycle arrest via up-regulation of cyclin-dependent kinase inhibitor p21. These collected data clearly demonstrated that peptides KT2 and RT2 offered great potential in the treatment of human colon cancer cells. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย