| Research Title |
Leucocyte recruitment associates dextran sulfate sodium-induced ulcerative colitis in ICR mice |
| Date of Distribution |
22 May 2019 |
| Conference |
| Title of the Conference |
The 4th MSU International Veterinary Conference |
| Organiser |
Faculty of Veterinary Sciences, Mahasarakham University |
| Conference Place |
Animal Hospital, Faculty of Veterinary Sciences, Mahasarakham Universit |
| Province/State |
มหาสารคาม |
| Conference Date |
21 May 2019 |
| To |
22 May 2019 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
23 |
| Editors/edition/publisher |
|
| Abstract |
Background: Ulcerative colitis (UC) is an idiopathic inflammatory disorder. Pathophysiological mechanism of UC remains unclear; it could be characterized by inflammatory response in colon. A pro-inflammatory cytokine, tumor necrosis factor alpha (Tnf-α), plays a key role to activate leucocyte recruitment via enhancing adhesion molecules, namely intercellular adhesion molecule 1 (Icam-1) and vascular cell adhesion molecule 1 (Vcam-1), and a chemokine monocyte chemoattractant protein 1 (Mcp-1). This study focused on how inflammatory pathway associated dextran sulfate sodium (DSS)-induced UC in outbred ICR mice.
Methods: A 21% (w/v) aqueous solution of DSS (MW 40 kDa) was prepared. Male ICR mice (6 weeks old) were orally administrated a 0.25 mL-aliquot of DSS solution 4 times a day for 4 and 7 consecutive days. The control mice were simply given sterile water. At the end of UC induction, colons were collected to examine expressions of Tnf-α, Mcp-1, Icam-1, and Vcam-1 mRNA by RT-qPCR.
Results:
DSS significantly elevated the expression of Tnf-α and Mcp-1 mRNA compared to the control at both day 4 and day 7 of the UC induction. The mRNA levels of Icam-1 and Vcam-1 were up-regulated in the DSS-treated mice with a significant difference from the controls on the day 7 of induction.
Conclusion:
These findings revealed the activation of leucocyte recruitment as the pathophysiological mechanism, at least in part, of the DSS-induced UC in ICR mice.
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
The best oral presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
คณะสัตวแพทยศาสตร์ มหาวิทยาลัยมหาสารคาม |
| Date of awarding |
22 พฤษภาคม 2562 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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