2012 ©
             Publication
Journal Publication
Title of Article Influences of Ammonium Nitrate on Growth and Inulin Content of Jerusalem Artichoke (Helianthus tuberosus L.) in Vitro 
Date of Acceptance 28 January 2019 
Journal
     Title of Journal Naresuan University Journal: Science and Technology 
     Standard  
     Institute of Journal Naresuan University  
     ISBN/ISSN 0858-7418 
     Volume 27 
     Issue 3  
     Month July-September
     Year of Publication 2019 
     Page 73-81 
     Abstract Jerusalem artichoke (Helianthus tuberosus L.) consists of high inulin and fructose, useful for the pharmaceuticals and chemical industries. Therefore, previous researchers have studied on the micropropagation of Jerusalem artichoke from seeds, nodes, and young leaves; effects of sugar and plant growth regulators on culture and inulin content in Jerusalem artichoke; and microtuber propagation. However, studies on the effects of ammonium nitrate (NH4NO3) on growth and biosynthesis of inulin in Jerusalem artichoke are scarce at present. Therefore, the objective of this work was to evaluate the effect of NH4NO3 on the growth of Jerusalem artichoke and its inulin content in vitro. Auxiliary meristems from the field-grown plants were cultured on MS medium containing 0, 825, 1,650, and 3,300 mg/L NH4NO3. The inulin content of plantlets was analyzed by HPLC-RID after subculturing every 4 weeks for 5 times. The results showed that shoot length, node number, leaf number, leaf width, leaf length, fresh weight, and dry weight were maximum in 3,300 mg/L NH4NO3. While shoot number and root number were best in 1,650 mg/L NH4NO3. Furthermore, the study found maximum inulin content on MS containing 1,650 mg/L NH4NO3 and higher hyperhydricity in higher concentrations of NH4NO3. Overall, MS containing 1,650 mg/L NH4NO3 showed a positive effect on vegetative growth, hyperhydricity, and inulin production of Jerusalem artichoke. It can be used as an alternative approach for propagation and inulin production from Jerusalem artichoke.  
     Keyword Ammonium nitrate, HPLC – RID, Inulin, In vitro, Jerusalem artichoke 
Author
585030045-8 Miss PHUANGPHA PUNHA [Main Author]
Agriculture Master's Degree

Reviewing Status ไม่มีผู้ประเมินอิสระ 
Status ตีพิมพ์แล้ว 
Level of Publication นานาชาติ 
citation false 
Part of thesis true 
Attach file
Citation 0