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Journal Publication
Title of Article Validation of Cell-Based Assay for Quantification of Sesamol Uptake and Its Application for Measuring Target Exposure 
Date of Acceptance 28 September 2019 
     Title of Journal Molecules 
     Standard ISI 
     Institute of Journal MDPI AG (Basel, Switzerland) 
     ISBN/ISSN doi.org/10.3390/molecules24193522 
     Volume 24 
     Issue 19 
     Month September
     Year of Publication 2019 
     Page 3522 
     Abstract The intracellular drug concentration is needed for determination of target exposure at the site of action regarding its pharmacological action and adverse effects. Sesamol is an antiproliferative molecule from Sesamum indicum with promising health benefits. We present a method for measuring the intracellular sesamol content using reverse-phase HPLC with a UV diode array in melanoma cells. Sesamol was completely resolved by isocratic elution (4.152 ± 0.008 min) with methanol/water (70%, v/v) through a 30 °C, 5-µm C-18 column and detection at 297 nm. The present assay offers high sensitivity, fast elution, and an accurate and linear nominal concentration range of 10–1000 ng/mL (R2 = 0.9972). The % accuracy of the sesamol quality control sample was −3.36% to 1.50% (bias) with a 0.84% to 5.28% relative standard deviation (RSD), representing high repeatability and high reproducibility. The % recovery was 94.80% to 99.29%, which determined that there was no loss of sesamol content during the sample preparation. The validated method was applied to monitor intracellular sesamol concentration after treatment from 5 min to 24 h. The remaining intracellular sesamol content was correlated with its antiproliferative effect (R2 = 0.9483). In conclusion, this assay demonstrated low manipulation, quick elution, and high sensitivity, precision, accuracy, and recovery, and it was successfully applied to the quantification of sesamol in target cells. 
     Keyword Sesamum indicum; Pedaliaceae; sesamol; HPLC; method validation; melanoma; cell-based assay; intracellular concentration 
607150007-3 Mr. TARAPONG SRISONGKRAM [Main Author]
Pharmaceutical Sciences Doctoral Degree

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