| Title of Article |
Role of L-Type Amino Acid Transporter 1 (LAT1) for
the Selective Cytotoxicity of Sesamol in Human
Melanoma Cells |
| Date of Acceptance |
25 October 2019 |
| Journal |
| Title of Journal |
Molecules |
| Standard |
ISI |
| Institute of Journal |
MDPI AG (Basel, Switzerland) |
| ISBN/ISSN |
doi.org/10.3390/molecules24213869 |
| Volume |
24 |
| Issue |
21 |
| Month |
November |
| Year of Publication |
2019 |
| Page |
3869 |
| Abstract |
Sesamol is effective against melanoma cells with less damage to normal cells. The underlying
selective cytotoxicity of sesamol in melanoma vs. non-cancerous cells is undefined. Melanoma cells
differ from normal cells by over-expression of the L-type amino acid transporter 1 (LAT1). We sought
to clarify the transport mechanism on selective cytotoxicity of sesamol in melanoma cells. A human
melanoma cell line (SK-MEL-2) and African monkey epithelial cell line (Vero) were used to study the
cellular uptake and cytotoxicity of sesamol. The intracellular concentration of sesamol was quantified
by UV-HPLC. The cytotoxicity was determined by neutral red uptake assay. Sesamol showed a higher
distribution volume and uptake clearance in SK-MEL-2 than Vero cells. Sesamol was distributed
by both carrier-mediated and passive transport by having greater carrier-mediated transport into
SK-MEL-2 cells than Vero cells. Higher mRNA expression and function of LAT1 over LAT2 were
evident in SK-MEL-2 cells compared to Vero cells. Sesamol uptake and sesamol cytotoxicity were
inhibited by the LAT1 inhibitor, suggesting LAT1 had a role in sesamol transport and its bioactivity in
melanoma. The LAT1-mediated transport of sesamol is indicative of how it engages cytotoxicity in
melanoma cells with promising therapeutic benefits |
| Keyword |
Sesamum indicum; Pedaliaceae; sesamol; L-type amino acid transporter 1; melanoma; uptake transport; selective cytotoxicity |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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