| Research Title |
Fourier transform infrared (FTIR) microspectroscopy
distinguished between adherent and multicellular tumor
spheroid cell culture in human malignant melanoma cells |
| Date of Distribution |
1 May 2019 |
| Conference |
| Title of the Conference |
การประชุมกลุ่มผู้ใช้ประโยชน์แสงซิงโครตรอน ประจำปี 2562 |
| Organiser |
ส่วนบริการผู้ใช้ ฝ่ายกลยุทธ์และพัฒนาธุรกิจองค์กร สถาบันวิจัยแสงซินโครตรอน (องค์การมหาชน |
| Conference Place |
โรงแรม เดอะ สุโกศล กรุงเทพ |
| Province/State |
กรุงเทพมหานคร |
| Conference Date |
1 May 2019 |
| To |
1 May 2019 |
| Proceeding Paper |
| Volume |
2019 |
| Issue |
1 |
| Page |
20 |
| Editors/edition/publisher |
ส่วนบริการผู้ใช้ ฝ่ายกลยุทธ์และพัฒนาธุรกิจองค์กร สถาบันวิจัยแสงซินโครตรอน (องค์การมหาชน) |
| Abstract |
Fourier transform infrared (FTIR) microspectroscopy has been promising to discriminate the
difference of cell samples with non-destructive, fast, high sensitivity and cost-effective ability [1-3].
Three-dimensional (3D) tumor spheroid has been engaged in drug discovery and drug development due
to it is mimicking in vivo tumor physiology model more than the conventional two dimensional (2D)
adherent cell model [4]. Thus, we used the FTIR microspectroscopy as a tool to evaluate the
differentiation between 2D and 3D cell models. In this study the cell models were developed from the
human melanoma cell line (SK-MEL-2). The 2D and 3D cells were collected and spotted on a barium
fluoride window. The IR spectra were collected and measured in transmission mode over a
measurement range of 3000 – 900 cm-1 by FTIR microspectroscopy. The average primary absorbance
spectra of the cells from 3D spheroid was clearly discriminated from 2D cells without performing the 2nd
derivative. This result was confirmed by the cluster analysis. The PCA and loading plot revealed that
the respective lipid (2945, 2848 cm-1), amide I (1660, 1623 cm-1), amide II (1507 cm-1) and nucleic acid
(1091 cm-1) had higher peak height that were contributed to discrimination between 2D and 3D cell
models. In addition, the integrated area under the original spectra of amide I and nucleic acid regions
of the 3D model were significantly lower than 2D model. However, the amide II and lipid (C=O) regions
were significantly and non-significantly higher than 2D model, respectively. In conclusion, the FTIR
microspectroscopy is the effective tool to discriminate the spheroid and adherent cell models. It can be
applied for the identification and for quality control of multicellular spheroid model compared to 2D cell
model alternatively to the biochemical technique. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
Poster presentation ในระดับดีมาก |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
ส่วนบริการผู้ใช้ ฝ่ายกลยุทธ์และพัฒนาธุรกิจองค์กร สถาบันวิจัยแสงซินโครตรอน (องค์การมหาชน |
| Date of awarding |
1 พฤษภาคม 2562 |
| Attach file |
|
| Citation |
0
|
|
|
Publication
Journal Publication
ไทย