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Journal Publication
Research Title Fourier transform infrared (FTIR) microspectroscopy distinguished between adherent and multicellular tumor spheroid cell culture in human malignant melanoma cells 
Date of Distribution 1 May 2019 
Conference
     Title of the Conference การประชุมกลุ่มผู้ใช้ประโยชน์แสงซิงโครตรอน ประจำปี 2562 
     Organiser ส่วนบริการผู้ใช้ ฝ่ายกลยุทธ์และพัฒนาธุรกิจองค์กร สถาบันวิจัยแสงซินโครตรอน (องค์การมหาชน 
     Conference Place โรงแรม เดอะ สุโกศล กรุงเทพ 
     Province/State กรุงเทพมหานคร 
     Conference Date 1 May 2019 
     To 1 May 2019 
Proceeding Paper
     Volume 2019 
     Issue
     Page 20 
     Editors/edition/publisher ส่วนบริการผู้ใช้ ฝ่ายกลยุทธ์และพัฒนาธุรกิจองค์กร สถาบันวิจัยแสงซินโครตรอน (องค์การมหาชน) 
     Abstract Fourier transform infrared (FTIR) microspectroscopy has been promising to discriminate the difference of cell samples with non-destructive, fast, high sensitivity and cost-effective ability [1-3]. Three-dimensional (3D) tumor spheroid has been engaged in drug discovery and drug development due to it is mimicking in vivo tumor physiology model more than the conventional two dimensional (2D) adherent cell model [4]. Thus, we used the FTIR microspectroscopy as a tool to evaluate the differentiation between 2D and 3D cell models. In this study the cell models were developed from the human melanoma cell line (SK-MEL-2). The 2D and 3D cells were collected and spotted on a barium fluoride window. The IR spectra were collected and measured in transmission mode over a measurement range of 3000 – 900 cm-1 by FTIR microspectroscopy. The average primary absorbance spectra of the cells from 3D spheroid was clearly discriminated from 2D cells without performing the 2nd derivative. This result was confirmed by the cluster analysis. The PCA and loading plot revealed that the respective lipid (2945, 2848 cm-1), amide I (1660, 1623 cm-1), amide II (1507 cm-1) and nucleic acid (1091 cm-1) had higher peak height that were contributed to discrimination between 2D and 3D cell models. In addition, the integrated area under the original spectra of amide I and nucleic acid regions of the 3D model were significantly lower than 2D model. However, the amide II and lipid (C=O) regions were significantly and non-significantly higher than 2D model, respectively. In conclusion, the FTIR microspectroscopy is the effective tool to discriminate the spheroid and adherent cell models. It can be applied for the identification and for quality control of multicellular spheroid model compared to 2D cell model alternatively to the biochemical technique. 
Author
607150007-3 Mr. TARAPONG SRISONGKRAM [Main Author]
Pharmaceutical Sciences Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference ชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding true 
     Award Title Poster presentation ในระดับดีมาก 
     Type of award รางวัลด้านวิชาการ วิชาชีพ 
     Organiser ส่วนบริการผู้ใช้ ฝ่ายกลยุทธ์และพัฒนาธุรกิจองค์กร สถาบันวิจัยแสงซินโครตรอน (องค์การมหาชน 
     Date of awarding 1 พฤษภาคม 2562 
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