| Research Title |
DEMONSTRATION OF IN-HOUSE BEAD-BASED IMMUNOASSAY FOR HUMAN SERUM ALBUMIN DETECTION BASED ON MICROFLUIDICS FLOW CYTOMETRY |
| Date of Distribution |
6 October 2020 |
| Conference |
| Title of the Conference |
The 46th International Conference on Science, Technology and Technology-based Innovation (STT 46) |
| Organiser |
The Science Society of Thailand under the Patronage of His Majesty the King |
| Conference Place |
มหาวิทยาลัยรามคำแหง |
| Province/State |
กรุงเทพมหานคร |
| Conference Date |
5 October 2020 |
| To |
7 October 2020 |
| Proceeding Paper |
| Volume |
46 |
| Issue |
1 |
| Page |
625 |
| Editors/edition/publisher |
|
| Abstract |
Human serum albumin (HSA) is the most abundant protein in the blood vessel.
It serves as a valuable biomarker for a wide range of health conditions such as poor nutritional status and kidney conditions. HSA is one of the key parameters in various medical diagnostics and health checkup programs. It can be detected using various laboratory-based approaches, but very few aimed for point-of-care detection purpose. Herein, we demonstrate an in-house bead-based fluorescent immunoassay platform, the universal form that can be further applied to use in microfluidics flow cytometry-based point-of-care devices. In this research, polyclonal human serum albumin antibody is successfully coupling onto 10 µm fluorescent beads at ratio 20 µg per 5.5 x 104 beads. 0.29 mg/mL of monoclonal human serum albumin antibody was successfully conjugated with Pacific blue™ dye which yields 89.04 %. The standard curve presented high sensitivity with the linear range of 190 – 6,670 ng/mL and the limit of detection 160 ng/mL. These results demonstrated that quantitative detection of human serum albumin could be performed using the bead-based immunoassay platform. The experimental conditions would be a model for further optimize and apply on microfluidics flow cytometry-based point-of-care devices. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย