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Publication
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| Title of Article |
eDNA removal enhances D-LL-31 activity against biofilms of
bacteria isolated from chronic rhinosinusitis patients |
| Date of Acceptance |
22 November 2020 |
| Journal |
| Title of Journal |
Biofouling |
| Standard |
ISI |
| Institute of Journal |
Taylor & Francis |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2020 |
| Page |
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| Abstract |
Chronic rhinosinusitis (CRS) is a chronic infection of the nasal cavity and paranasal sinuses associated with the presence of a microbial biofilms. Extracellular DNA (eDNA) is an important component of the biofilm matrix. Antimicrobial peptides (AMPs) are natural peptides with the ability to kill microorganisms. D-LL-31, a synthetic variant of the AMP cathelicidin with increased resistance to proteolytic breakdown. In this study we show for 3 clinical CRS isolates, that treatment of 24 h biofilms with DNase I enhanced the antimicrobial activity of D-LL-31. Conversely, co-incubation of D-LL-31 at IC50 value with exogenous DNA resulted in reduced antimicrobial activity. DNase I alone did not show antimicrobial activity against the tested isolates but caused dispersal of established biofilm. Hence, presence of eDNA in the biofilm matrix reduced AMP-mediated killing. These results suggest that combination therapy with proteolysis resistant AMP D-LL-31 and DNase could be considered for effective treatment of CRS. |
| Keyword |
Bactericidal activity, D-enantiomer peptide, D-LL-31, eDNA, DNase I, Chronic rhinosinusitis |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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