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Publication
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| Title of Article |
Optimization of vitamin C concentration to boost PMN function in healthy individuals |
| Date of Acceptance |
9 June 2020 |
| Journal |
| Title of Journal |
Archives of Allied Health Sciences (Arch AHS) |
| Standard |
TCI |
| Institute of Journal |
Faculty of Associated Medical Sciences, Khon Kaen University |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2021 |
| Page |
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| Abstract |
Vitamin C is an antioxidant agent and a promoter of biological functions including immune cells,
7 particularly of neutrophils or polymorphonuclear (PMN) cells, which play a vital role in bacterial infection.
8 The data of vitamin C impacting on PMN function during bacterial phagocytosis in healthy individuals are
9 limited. This study aimed to investigate the optimal concentration and conditions of vitamin C that could
10 enhance PMN phagocytosis and oxidative burst in healthy adult ex vivo using whole blood assay. Whole
11 blood samples from healthy individuals were pre-incubated without and with vitamin C in various
12 concentrations before co-culture with Staphylococcus aureus and detection of the PMN function by flow
13 cytometry. Vitamin C at 20 mM significantly increased phagocytosis (p-value=0.03) and had a trend to
14 significantly induce oxidative burst (p-value=0.06, p-trend=0.04) at 15 minutes after the bacteria exposure
15 (n=3), compared to untreated, while vitamin C concentration of 30 mM induced significant increase both
16 phagocytosis (p-value=0.04) and oxidative burst (p-value=0.02). We further investigated the boosting effect
17 of vitamin C at 20 mM, as a minimal concentration that can boost phagocytosis, on PMN function in
18 additional 17 healthy participants and found that both phagocytosis and oxidative burst of PMN were
19 significantly increased (p-value<0.0001). In conclusion, vitamin C at 20 mM can enhance PMN function in
20 healthy adults within 15 minutes after exposure of the bacteria. This condition may be benefit to PMN to
21 eliminate bacterial infection rapidly. Nevertheless, further research in the clinical trial and underlying
22 mechanisms await further studies. |
| Keyword |
Vitamin C, PMN function, Neutrophils, Phagocytosis, Oxidative burst |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
ชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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