| Research Title |
Competitive uptake of sesamol with L-amino acids in melanoma cells |
| Date of Distribution |
23 May 2019 |
| Conference |
| Title of the Conference |
โครงการติดตามผลงานวิจัย ของนิสิตและนักศึกษา คปก. RGJ-University Forum (ภาคตะวันออกเฉียงเหนือ) ประจําปีงบประมาณ 2562 |
| Organiser |
โครงการปริญญาเอกกาญจนาภิเษก (RGJ-Ph.D. Thailand research fund) |
| Conference Place |
โรงแรมอวานี ขอนแก่น โฮเทล แอนด์ คอนเวนชั่น เซ็นเตอร์ |
| Province/State |
ขอนแก่น |
| Conference Date |
23 May 2019 |
| To |
23 May 2019 |
| Proceeding Paper |
| Volume |
2019 |
| Issue |
1 |
| Page |
1 |
| Editors/edition/publisher |
ศ.ดร.โสพิศ วงศ์คา และ ศ.ดร.วันชัย มาลีวงษ์ |
| Abstract |
Sesamol—an active compound found in Sesame indicum—has strong antiproliferation against melanoma with no cytotoxicity to noncancerous Vero cells. Sesamol is less lipophilic, but how sesamol was uptaken intracellularly has not yet been elucidated. Since melanoma was reported to overexpression of the L-type amino acid transporter 1 (LAT1). Thus the competitive uptake between sesamol, the specific LAT1 substrates and the LAT1 inhibitor were evaluated. The SK-MEL-2 human malignant melanoma cell line was used for evaluation of the uptake of L-dopa in the presence and the absence of LAT1 substrates (i.e., L-leucine, and L-alanine), the LAT1 inhibitor (i.e., 2-amino-2- norbornanecarboxylic acid; BCH), and sesamol. The intracellular uptake of sesamol was also determined with and without L-leucine, L-alanine, and BCH. Sesamol and L-dopa contents in cells were quantified by the validated HPLC analysis. The significant decrease of intracellular uptake of L-dopa in the presence of BCH compared to the control confirmed the existence of LAT1 function at the cell membrane of SK-MEL-2 cells. The %reduction of L-dopa uptake in the presence of the other substrates and sesamol indicated that sesamol was competitively uptaken with L-dopa similar to the other LAT1 substrates. When consideration of the sesamol uptake intracellularly, the uptake of sesamol was significantly reduced in the presence of the LAT1 substrates and in the presence of BCH compared with the control. This study identifies the LAT1 as a mean of specialized uptake transportation of sesamol across the SK-MEL-2 melanoma cells. The underlined intracellular transportation pathway of sesamol indicates how sesamol engages its bioactivity in melanoma cells under the physiological condition with promising clinical benefit. |
| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย