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Publication
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| Title of Article |
CagA+ Helicobacter pylori infection and N-nitrosodimethylamine administration induce cholangiocarcinoma development in hamsters |
| Date of Acceptance |
5 May 2021 |
| Journal |
| Title of Journal |
Helicobacter |
| Standard |
SCOPUS |
| Institute of Journal |
John Wiley & Sons Ltd |
| ISBN/ISSN |
DOI: 10.1111/hel.12817 |
| Volume |
2021 |
| Issue |
Vol 27 |
| Month |
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| Year of Publication |
2021 |
| Page |
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| Abstract |
Background: Helicobacter pylori (HP) has been detected in the hepatobiliary tract of cholangiocarcinoma (CCA) patients in regions both endemic and non-endemic for Opisthorchis viverrini (OV) infection. However, whether H. pylori infection promotes CCA development remains unknown. We investigated CCA development in hamsters induced by a combination of infection with H. pylori and administration of N-nitrosodimethylamine (NDMA) and compared findings with those in an OV plus NDMA group.
Materials and Methods: Eighty-five hamsters were divided into four groups: (1) normal, (2) administered NDMA, (3) infected with cagA+ H. pylori and administered NDMA (HN group), and (4) infected with OV and administered NDMA (ON group). Animals were euthanized at 3 and 6 months post-infection. Histopathological changes of liver and the expression of markers associated with carcinogenesis were studied.
Results: At 3 months post-infection (p.i.), cholangitis and lymphoid follicles without tumor appearance were noted in the HN group, whereas extensive fibrosis was seen in members of the ON group, 10% of which had developed tumors. At 6 months p.i., 10% of hamsters administered NDMA alone had developed CCA, whereas in the HN and ON groups, 20% and 60% of hamsters, respectively, had developed CCA. Cytokeratin-19 ( CK-19) expression was observed in the CCA tissues of both the HN and the ON groups, confirming the bile-duct origin of the CCA cells. CCA development in the HN group might be inflammation-mediated, as suggested by overexpression of HMGB1, PCNA, IL-8, and 8-OxodG in CCA tissues.
Conclusion: cagA+ H. pylori infection and carcinogen intake can induce CCA development with slow progression.
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| Keyword |
Cholangiocarcinoma, Helicobacter pylori, Opisthorchis viverrini, histopathological study, risk factor, the animal model |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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