| Research Title |
Detection of Japanese Encephalitis Virus in Culex mosquitoes in Ubon Ratchathani province, Thailand |
| Date of Distribution |
28 May 2021 |
| Conference |
| Title of the Conference |
การประชุมวิชาการนำเสนอผลงานวิจัยระดับบัณฑิตศึกษาแห่งชาติ ครั้งที่ 52 |
| Organiser |
บัณฑิตวิทยาลัย มหาวิทยาลัยบูรพา |
| Conference Place |
บัณฑิตวิทยาลัย มหาวิทยาลัยบูรพา |
| Province/State |
ชลบุรี |
| Conference Date |
28 May 2021 |
| To |
28 May 2021 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
1030-1039 |
| Editors/edition/publisher |
บัณฑิตวิทยาลัย มหาวิทยาลัยบูรพา |
| Abstract |
Japanese encephalitis virus (JEV) is mosquito-borne flavivirus that causes neurological disorder in humans and has Culex mosqui¬toes as the primary vector for transmission. Although Thailand has good Japanese encephalitis (JE) control programs including national immunization program and vector control, JE patients have been found annually. The previous data in 2018 to 2019 showed that Ubon Ratchathani province has the highest morbidity rate of JE. To know an existing JEV in this region, this study investigated JEV infection in Culex mosquitoes that were collected from the selected households from one urban area (Don Yung) and one rural area (Phon Duan), in Ubon Ratchathani province from January to December 2019 by using the Prokopack mosquito aspirator. Mosquitoes were pooled and homogenized by using the micro pestle with L-15 media. RNA was extracted from supernatants of mosquito pool samples and synthesized cDNA by SuperScriptIII first-strand synthesis system. Specific primers were created and optimized for sensitivity of detection. The quantified cDNA were used to detect E and partial NS1 genes of JEV all genotypes by real-time reverse transcriptase polymerase chain reaction. The result showed that a total of 335 RNA samples from mosquito pools were good qualified. The detection of the JEV gene was negative. These data provide information about no existing JEV in the main vector in the study sites suggesting serosurveillance of JEV in secondary hosts or infected asymptomatic persons should be done to understand the circulating of this virus in the endemic area. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย