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Publication
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| Title of Article |
Molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand |
| Date of Acceptance |
17 August 2021 |
| Journal |
| Title of Journal |
Veterinary world journal |
| Standard |
SCOPUS |
| Institute of Journal |
India |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2021 |
| Page |
19 |
| Abstract |
Background: Anaplasma platys is a blood parasite that infects platelets causing thrombocytopenia. Rhipicephalus sanguineus ticks are believed to transmit A. platys. For the identificaion of A. platys, nested PCR is proved as an effective diagnostic tool.
Aim: In this study molecular prevalence of A. platys infection in dogs was investigated for the first time in the Khon Kaen region of Thailand. The association between risk factors and A. platys infection was also evaluated.
Materials and methods: A total of 130 blood samples were collected from dogs in Khon Kaen, Thailand. DNA from the samples was extracted and nested PCR was applied for molecular examination. Platelet count and PCV (packed cell volume) levels were measured. Platelet counts were categorised into 4 grades: Non-thrombocytopenia (platelet >200,000 cell/µl), mild thrombocytopenia (platelet 150,000-200,000 cell/µl), moderate thrombocytopenia (platelet 100,000-150,000 cell/µl) and severe thrombocytopenia (platelet <100,000 cell/µl). Four categories for PCV level of >37%, 30-37%, 20-29%, and <20% were defined as non-anemia, mild anemia, moderate anemia, and severe anemia, respectively. DNA sequencing was analysed for similarity index.
Results: Among 130 samples, 9 (6.9%) were found positive for A. platys infection. There was an association between low platelet count and infection (p<0.05). PCV level was also associated with A. platys infection (p<0.05). DNA sequencing results of the 9 positive samples showed similarity to known sequences of A. platys with 99.36-100% nucleotide identities. These results suggested low genetic diversity in A. platys infecting dogs in the Khon Kaen area.
Conclusion: By amplifying 16S rRNA, Anaplasma platys infection was detected in the blood of Thai dogs. The identification of risk factors that may be associated with A. platys infection in dogs in Khon Kaen should be further investigated. Consideration of other related factors should be given such as the location and breeding, as well as the environmental characteristics of each locality. In addition, sampling a larger number of animals may demonstrate possible predictors for the positivity of A. platys in dogs in this region.
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| Keyword |
Anaplasma platys, thrombocytotropic anaplasmosis, molecular prevalence, nested PCR |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Attach file |
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| Citation |
0
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Journal Publication
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