|
|
Publication
|
| Research Title |
Analysis of Active Ingredients of Thai Propolis Extracts Using High Performance Liquid Chromatography |
| Date of Distribution |
17 November 2021 |
| Conference |
| Title of the Conference |
International Scientific Conference of the Dental Faculty Consortium of Thailand |
| Organiser |
Chiang Mai University |
| Conference Place |
Chiang Mai Unviversity |
| Province/State |
Chiang Mai, Thailand |
| Conference Date |
17 November 2021 |
| To |
19 November 2021 |
| Proceeding Paper |
| Volume |
18 |
| Issue |
2021 |
| Page |
268 |
| Editors/edition/publisher |
|
| Abstract |
Background: Propolis has been proven to have numerous beneficial effects in dentistry, such as an effective pulp-capping material, intra-canal medicament, and in preservation of periodontal ligament cells. These are attributed to flavonoids and phenolic compounds.
Objectives: To determine the presence of vicenin-2, vitexin, apigenin, and cinnamic acid in Thai propolis extracts using high performance liquid chromatography (HPLC); and, to analyze whether its concentration will be affected by the ethanolic concentration used in extraction and its source location.
Materials and Methods: Fifty milligrams of propolis from Nong Khai and Loei were extracted using either 100%,90%,80%, or 70% ethanol. These were analyzed using HPLC with a mobile phase gradient system of 10-100% acetonitrile in 0.05% aqueous ortho-phosphoric acid, flow rate of 0.8mL/min, and detection wavelength of 280nm. The concentration of the four components were compared according to location and ethanolic extraction using Two-way ANOVA with significance level at p<0.05.
Results: The source of propolis and the percentage of ethanol used in its extraction both have a significant role in the concentration of all active ingredients (p < 0.01). Vicenin-2 is significantly affected by each change in the percentage of ethanol extraction. Vitexin and cinnamic acid showed statistical difference between 100%,80% and 70% ethanolic extracts, respectively. Apigenin results in significant difference between 100%, 90%, and 70% ethanolic extracts only.
Conclusion: Propolis from Nong Khai have a significantly higher amount of the four active ingredients as compared to Loei samples. These ingredients have significantly higher concentrations in 70% ethanol extraction, as compared to higher concentrations of ethanol.
Keywords: Thai Propolis, High Performance Liquid Chromatography, Flavonoids, Phenolic Compounds
|
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
7
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย