| Research Title |
Melatonin Ameliorates the Hippocampal Neural Stem Cell Decline in the Adult Rats Receiving 5-fluorouracil |
| Date of Distribution |
7 September 2022 |
| Conference |
| Title of the Conference |
งานประชุมวิชาการ ประจำปี 2565 คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น ครั้งที่ 36 (ภายใต้หัวข้อ 50th Year MD KKU Moving Forward) |
| Organiser |
Faculty of Medicine |
| Conference Place |
Khon Kaen University |
| Province/State |
Khon Kaen |
| Conference Date |
7 September 2022 |
| To |
8 September 2022 |
| Proceeding Paper |
| Volume |
37 (Suppl.) September 2022 |
| Issue |
1 |
| Page |
- |
| Editors/edition/publisher |
ศ.พญ.สุวรรณา อรุณพงค์ไพศาล |
| Abstract |
Background and objective: Melatonin has neuroprotective effects in the adult rat hippocampus, a brain region that adult neurogenesis occurs and relates to memory. 5-fluorouracil (5-FU), a chemotherapy, has been reported to have the ability to induce memory impairment caused by decreasing hippocampal neurogenesis and neuronal degeneration. The potential of melatonin on hippocampal neural stem cells in adult rats receiving 5-FU is important to be investigated. Therefore, the present study focused on the essential role of the hippocampal neural stem cells treated with melatonin in adult rats treated with 5-FU.
Methods: Rats were divided into four groups: vehicle, 5-FU, melatonin and melatonin+5-FU groups. Rats received 5-FU (25 mg/kg, i.v.) and melatonin (8 mg/kg, i.p.) treatments. Semi-thin brain sections were stained using toluidine blue to evaluate the stem cell niche quantification. Brain sections (40 µm) were stained using immunofluorescence technique to determine the expression of nestin and Sox2 in the subgranular zone of dentate gyrus in the hippocampus. The hippocampus was collected to measure protein expression of stem cell markers including nestin, Sox2 and Notch1 using Western blotting.
Results: The number of stem cell niches were significantly increased in the melatonin+5-FU group compared to the 5-FU group. The immunofluorescence staining of the melatonin+5-FU group demonstrates significant increases in nestin and Sox2-positive cells compared to the 5-FU group. Additionally, Western blotting revealed that animals in the melatonin+5-FU group were significantly up-regulated in nestin, Sox2 and Notch1 protein expressions compared to the 5-FU group.
Conclusions: Melatonin could improve the decline of stem cell niches induced by 5-FU. This finding supported that melatonin treatment could ameliorate decreases of neural stem cell number and nestin, Sox2 and Notch1 protein expressions in the adult rat hippocampus receiving 5-FU. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
รางวัลชมเชยการประกวดผลงานทางวิชาการ (การนำเสนอผลงาน Oral presentation กลุ่มนักศึกษาปริญญาโท-เอก) |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
Faculty of Medicine, Khon Kaen University |
| Date of awarding |
8 กันยายน 2565 |
| Attach file |
|
| Citation |
0
|
|
|
Publication
Journal Publication
ไทย