| บทคัดย่อ |
Sericin-alginate hydrogel formulations with purple waxy corn (Zea mays L.) cob extract
(PWCC) for topical anti-inflammatory application are developed and evaluated. The physical
properties such as viscosity, pH, and anthocyanin release are examined and in vitro anti-inflammatory
activities, such as NO inhibition and IL-6, IL-1β, TNF-α, iNOS, and COX-2 expression, are evaluated
in LPS-stimulated RAW 264.7 murine macrophages. The sericin-alginate hydrogel is prepared by
physical crosslinking through the ionic interaction of the polymers combined with anthocyanin from
PWCC at pH 6.5. The hydrogel formulation with 2.00% w/v sericin, 0.20% w/v alginate, and 0.15%
w/v PWCC (SN6) shows a suitable viscosity for topical treatment, the highest nitric oxide inhibition
(79.43%), no cytotoxicity, and reduced expression of IL-6, IL-1β, and TNF-α mediators. Moreover,
the SN6 formulation displays a sustained anthocyanin release over 8–12 h, which correlates with the
Korsmeyer–Peppas model. The FT-IR spectrum of SN6 confirmed interaction of the sericin polymer
with anthocyanins from PWCC via H-bonding by the shifted peak of amide I and amide III. In
addition, the anthocyanin is stable in sericin hydrogels under heating-cooling storage conditions.
Therefore, we suggest that this hydrogel formulation has potential as an anti-inflammatory agent.
The formulation will be further investigated for in vivo studies and clinical trials in the future. |
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