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Publication
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| Title of Article |
Moringa oleifera leaf extract ameliorates early stages of diabetic nephropathy in streptozotocin-induced diabetic rats |
| Date of Acceptance |
18 January 2023 |
| Journal |
| Title of Journal |
Journal of Applied Pharmaceutical Science |
| Standard |
SCOPUS |
| Institute of Journal |
MediPoeia |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Year of Publication |
2023 |
| Page |
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| Abstract |
Diabetic nephropathy (DN) is a long-term complication of diabetes mellitus. The characteristic of early-stage DN is glomerular hyperfiltration that has been linked to renal fibrosis. In the present study, we investigated the effect of Moringa oleifera (M. oleifera) leaf extract on DN in streptozotocin (STZ)-induced diabetic nephropathy rats. Rats were injected with 50 mg/kg STZ to establish the DN model. Four weeks after receiving an injection of STZ, DN rats were administered distilled water, M. oleifera leaf extract (100 or 200 mg/kg body weight/day), dapagliflozin (1 mg/kg body weight/day) or combinations of these for a further 8 weeks. DN rats exhibited significantly increased blood glucose (504.00±28.41 mg/dL), proteinuria (192.85±41.23 mg/24 h), albuminuria (6.68±1.54 mg/24 h), BUN (47.14±5.18 mg/dL) and creatinine clearance (5.64±.35 ml/min) (p<0.05) together with significantly increased MDA, and decreased SOD and catalase (p<0.05). Administration of M. oleifera leaf extract could significantly reduce the high blood glucose, impaired renal function and oxidative stress parameters of DN rats (p<0.05). Histological examination of kidneys showed a thickening of the glomerular basement membrane and an increase in the mesangial matrix, all of these pathological changes were improved by M. oleifera leaf extract administration. The mRNA expression of transforming growth factor beta1 (TGF-β1) and collagen type IV were significantly increased in the kidney tissue of DN rats, but were significantly down-regulated in M. oleifera leaf extract-treated rats (p<0.05). M. oleifera leaf extract could alleviate diabetic nephropathy plausibly due to its activities in reducing blood glucose, oxidative stress, and fibrosis formation by down-regulating the expression of TGF-β1 and collagen type IV genes. M. oleifera leaf extract may be useful as an alternative or supplementary medicine for treatment of diabetic nephropathy. |
| Keyword |
Diabetic nephropathy, Moringa oleifera, Streptozotocin, Renal fibrosis, Renal hyperfiltration |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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