| Title of Article |
Recombinase polymerase amplification (RPA) for human male determination from semen |
| Date of Acceptance |
5 May 2022 |
| Journal |
| Title of Journal |
Asia-Pacific Journal of Science and Technology |
| Standard |
SCOPUS |
| Institute of Journal |
Khon Kaen University,Research and Technology Transfer Affairs Division |
| ISBN/ISSN |
2539-6293 |
| Volume |
28 |
| Issue |
02 |
| Month |
March-April |
| Year of Publication |
2023 |
| Page |
|
| Abstract |
Recombinase polymerase amplification (RPA) is a simple, rapid, highly effective, specific, and sensitive
technique used for amplification of specific DNA regions at a constant, low temperature (between 37 °C and
42 °C) without using a thermal cycler. The RPA products can be detected using agarose gel electrophoresis. This
research developed primers specific to the SRY (sex-determining region Y) gene in RPA assay. RPA primers were
designed using parameters according to the instruction manual (TwistDxTM) and Primer-BLAST software based
on the human SRY gene (accession number: JQ811934.1) obtained from the GenBank database. The designed
primers were further tested using RPA at 39 °C for 20 min. The results revealed that the set of developed RPA
primers could detect all male DNA from human semen samples. Sensitivity analysis showed that the detection
limit was determined to be 0.01 ng for template DNA. Therefore, the RPA primers designed in this research are
an alternative valuable tool for sex determination as a part of biological evidence in forensic casework. |
| Keyword |
Male-specific marker, Recombinase Polymerase Amplification (RPA), SRY |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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