| Title of Article |
Establishment of in-house telomere length measurement using qPCR |
| Date of Acceptance |
23 November 2022 |
| Journal |
| Title of Journal |
Archives of Allied Health Sciences |
| Standard |
TCI |
| Institute of Journal |
คณะเทคนิคการแพทย์ มหาวิทยาลัยขอนแก่น |
| ISBN/ISSN |
|
| Volume |
2022 |
| Issue |
3 |
| Month |
กันยายน-ธันวาคม |
| Year of Publication |
2022 |
| Page |
44-52 |
| Abstract |
Telomere is a nucleoprotein complex at the ends of chromosomes which can be used as a biomarker for aging and health condition. The gold standard method for telomere length measurement is quite a complication. Recently, quantitative polymerase chain reaction (qPCR) is a widely used method for molecular study with high throughput and cost-effectiveness. This study aimed to develop the in-house qPCR for estimating telomere length in kilobase in healthy samples. The in-house qPCR was established for the telomere gene and 36B4 gene. The analytical performance was verified prior to applying to 190 healthy participants, 139 females and 51 males. The telomere length (kilobase) was calculated and compared to the reference value. The results for telomere length by in-house qPCR method were 7.48 ± 1.78 kb for males and 7.53 ± 1.45 kb for females with the range of 4.66 – 10.69 kb and 4.03 – 11.50 kb, respectively. A total of 190 participants showed moderate correlation with a reference value at R2 = 0.5672. The Bland-Altman analysis from two different methods showed only 5.26% (10 out of 190) were out of ± 25% bias in females. The in-house qPCR was successfully demonstrated for telomere length measurement with an acceptable performance compared to the reference values. However, the validation in more clinical samples should be performed in further study. |
| Keyword |
Telomere length; Chronological age; Quantitative polymerase chain reaction (qPCR). |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
ชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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