| บทคัดย่อ |
Heteroresistance in MTB refers to the presence of distinct subpopulations of bacteria with vary-ing levels of antibiotic susceptibility within a population. Multidrug-resistant and rifampic-in-resistant TB are serious global health concerns. In this study, we aimed to determine the prev-alence of heteroresistance in MTB from sputum samples of new TB cases using Droplet Digital PCR mutation detection assays for katG and rpoB genes, which are commonly associated with re-sistance to isoniazid and rifampicin, respectively. We found that out of 79 samples, 9 (11.4%) ex-hibited mutations in katG and rpoB genes. INH mono-resistant TB, RIF mono-resistant TB, and MDR-TB samples constituted 1.3%, 6.3%, and 3.8% of new TB cases, respectively. Heteroresistance in katG, rpoB, and both genes were found in 2.5%, 5%, and 2.5% of total cases, respectively. Our results suggest that these mutations may have arisen spontaneously, as the patients had not yet received anti-TB drugs. ddPCR is a valuable tool for the early detection and management of DR-TB, as it can detect both mutant and wild-type strains in a population, enabling the detection of heteroresistance and MDR-TB. Overall, our findings highlight the importance of early detec-tion and management of DR-TB for effective TB control (in katG, rpoB, and katG/rpoB). |
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