|
|
Publication
|
| Title of Article |
Improvement of nutritive value of soybean meal by microbial hydrolysis with Bacillus subtilis Hs-2 for use as raw material in Nile Tilapia (Oreochromis niloticus) diet |
| Date of Acceptance |
18 January 2024 |
| Journal |
| Title of Journal |
Aquaculture Reports |
| Standard |
SCOPUS |
| Institute of Journal |
Elsevier |
| ISBN/ISSN |
23525134 |
| Volume |
35 |
| Issue |
2024 |
| Month |
April |
| Year of Publication |
2024 |
| Page |
101943 |
| Abstract |
Soybean meal (SBM) is the popular feed ingredient in fish diets due to its good quality protein. However, the SBM
has a low content of some amino acids and contains several anti-nutritional factors (ANFs) that interfere with
digestion, absorption, and utilization of nutrients in the diet. This study aimed to investigate the appropriate
processing conditions for improving the nutritional quality of SBM using microbial hydrolysis with Bacillus
subtilis Hs-2 under optimal temperature at 37 ◦C at different fermentation periods of 24, 36, and 48 h compared
to non-fermented SBM as a control. The results showed that Bacillus subtilis Hs-2 could grow exponentially during
0–24 h. After incubation with various fermentation periods, no significant difference (P > 0.05) existed between
the SBM hydrolysates at 24, 36, and 48 h. Moreover, crude protein contents increased by 6.43%, 6.86% and
7.66%, respectively, while protease inhibitor activity decreased by 8.40%, 10.66%, and 11.68%, respectively,
and protein digestibility increased by 35.36%, 43.29% and 44.82% respectively. The highest total amino acid
was found in SBM hydrolysate at 24 h (P < 0.05), whereas the fiber content decreased to the lowest in SBM
hydrolysate at 48 h (P < 0.05). The phytic acid content and degree of hydrolysis exhibited no significant difference among all experimental groups (P > 0.05), ranging from 2.20–2.33% and 6.21–6.67%, respectively.
Some metabolites such as isoleucine, tryptophan, oxaloacetic acid, succinate, acetylcholine, and cellobiose were
increased in SBM hydrolysate at 24 h. For SBM hydrolysate at 24 h, the results in the release of small peptides
may act as antimicrobial against the following pathogenic microorganisms: Escherichia coli, Acinetobacter baumannii, Salmonella spp., Vibrio parahaemolyticus, and others. Overall, the results of this study indicated that
soybean meal fermented with Bacillus subtilis Hs-2 has excellent potential to improve the nutritional value and
could usefully be a fish meal replacement in the Nile Tilapia diet. |
| Keyword |
Soybean meal, Microbial hydrolysis, Bacillus subtilis Hs-2, Nutritive value, and Nile Tilapia |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย