| Research Title |
Suppression of O-GlcNAc Transferase Enhances Drug
Sensitivity of Melanoma |
| Date of Distribution |
7 September 2023 |
| Conference |
| Title of the Conference |
งานประชุมวิชาการประจำปี คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Organiser |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Conference Place |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Province/State |
ขอนแก่น |
| Conference Date |
7 September 2023 |
| To |
8 September 2023 |
| Proceeding Paper |
| Volume |
Volume 38 (Supplement) September 2023 |
| Issue |
ISSN 28219724 (ONLINE) |
| Page |
16 - 22 |
| Editors/edition/publisher |
ศ.สุวรรณา อรุณพงค์ไพศาล/38/ศรีนครินทร์เวชสาร (Srinagarind Medical Journal) |
| Abstract |
Abstract
Background and Objective: O-GlcNAcylation is an important O-linked glycosylation of nucleocytoplasmic proteins with a single molecule of N-acetylglucosamine. Aberrant O-GlcNAcylation was found to promote the development and progression of cancer. In this study, we have explored the role of O-GlcNAcylation in melanoma progression.
Methods: O-GlcNAcylation in MNT-1, a malignant melanoma cell line, was suppressed by a specific siRNA against N-acetylglucosaminyltransferase (OGT), compared with siControl. Cell proliferation and drug sensitivity were measured by SRB assay. Western blotting and immunofluorescent staining were used to determine the level of O-GlcNAcylated proteins (OGP). The molecular mechanism was explored by Western blotting.
Results: The level of OGP in MNT-1 was drastically reduced after treatment with siOGT compared with control siRNA (siControl). Knockdown of OGT significantly suppressed the cell proliferation ability of MNT-1 via inhibition of the Akt signaling pathway. The drug sensitivity assay revealed the synergistic effect of siOGT with cisplatin against MNT-1 melanoma cells, suggesting the potential of using OGT suppression for cisplatin sensitization for melanoma treatment.
Conclusions: In conclusion, we have provided evidence to improve melanoma therapy via targeting O-GlcNAcylation, which is identified to be involved in melanoma cell proliferation and chemoresistance.
Keywords: O-GlcNAc transferase, O-GlcNAcylation, Drug-sensitivity, Melanoma
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
ชมเชย |
| Type of award |
รางวัลด้านกีฬาและสุขภาพ |
| Organiser |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Date of awarding |
8 กันยายน 2566 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย