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Publication
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| Research Title |
Effects of osmoprotectant on ethanol fermentation from sweet sorghum stem juice in very high
gravity condition by Saccharomyces cerevisiae under temperature stress |
| Date of Distribution |
29 November 2023 |
| Conference |
| Title of the Conference |
The 35th Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2023) “Sustainable Development through Bio-Circular Green (BCG) Economy Model” |
| Organiser |
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology (SUT) |
| Conference Place |
The Greenery Resort Khao Yai, Khao Yai National Park, |
| Province/State |
Nakhon Ratchasima |
| Conference Date |
13 March 2024 |
| To |
13 March 2024 |
| Proceeding Paper |
| Volume |
2023 |
| Issue |
1 |
| Page |
103 |
| Editors/edition/publisher |
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| Abstract |
Very high gravity (VHG) ethanol fermentation has several advantages for industrial applications because it can increase ethanol titer, which not only reduces energy consumption for ethanol distillation, but also reduces waste discharged from distillation system. However, substrate inhibition usually occurs under a VHG condition due to high osmotic pressure, especially fermentation under environmental stresses. The aim of this study was to improve VHG ethanol fermentation under temperature stress by osmoprotectant (40 mM KCl and 10 mM KOH) addition. Sweet sorghum stem juice (SSJ) containing total sugar, 267 g/L and urea, 3.24 g/L fermented by Saccharomyces cerevisiae NP01 (an initial cell, 1.32×108 cells/mL) was found to be the optimum condition for VHG ethanol fermentation at the optimum fermentation temperature (30ºC). The ethanol concentration (PE), ethanol productivity (QP), and sugar consumption (%SC) were 119.3 g/L, 2.49 g/L.h. and 91.8%, respectively, at 48 h. Under fermentation temperature stress at 25, 35, 37 and 39ºC, the PE values decreased to 105.94 (at 60 h), 110.8, 91.5 and 74.6 g/L at 36 h, respectively. When the osmoprotectant (KCl/KOH) was added in the SSJ medium, at the fermentation temperature of 25, 35 and 37ºC, the PE values increased ≈10, 4 and 13%, respectively compared to those without osmoprotectant. This might be due to the fact that the osmoprotectant had positive effects on yeast cells, resulting in higher cell viability. However, at too high fermentation temperature at 39ºC, KCl/KOH addition could not improve ethanol fermentation. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย