| Research Title |
Thermosensitive chitosan-based hydrogel encapsulating human adipose-derived stem cells for cartilage engineering |
| Date of Distribution |
11 June 2012 |
| Conference |
| Title of the Conference |
Bone and Cartilage: from Development to Human Diseases |
| Organiser |
Cold Spring Harbor Asia |
| Conference Place |
Suzhou Dushu Lake Conference Center |
| Province/State |
SIP/ Suzhou, Jiangsu Province, China |
| Conference Date |
11 June 2012 |
| To |
15 June 2012 |
| Proceeding Paper |
| Volume |
2012 |
| Issue |
1 |
| Page |
28 |
| Editors/edition/publisher |
|
| Abstract |
Background: Articular cartilage has limited potential for repair. Current clinical treatments often result in fibrocartilage which has inferior mechanical property. Cartilage engineering is viewed as a promising therapy based on the utilization of progenitor cells and scaffolds.
Objective: To assess the ability of the developed thermosensitive chitosan-based hydrogel to support chondrogenesis of human adipose-derived stem cells (hADSCs).
Methods: The thermosensitive chitosan-based hydrogel was developed in our laboratory by blending chitosan (C) and starch (S) with -glycerol phosphate (GP). The morphology and biocompatibility of C/S/GP hydrogels encapsulating hADSCs were assessed. The chondrogenic differentiation of hADSCs was induced by culturing the hADSCs-encapsulated C/S/GP hydrogels in chondrogenic medium with TGF-β1 for 4 weeks, and the chondrogenic markers were evaluated by mRNA expression of cartilage extracellular matrix components.
Results: The C/S/GP hydrogel showed liquid state at room temperature and gel state at 37C. Scanning electron microscope (SEM) images revealed interconnected pore structure having pore sizes in the range of 30-120 m. The biocompatibility of hADSCs and C/S/GP hydrogel was demonstrated by cell adhesion and cell proliferation, using SEM image and XTT assay respectively. RT-PCR showed an up-regulation in aggrecan, type II and type X collagen in the hydrogel culture system comparable to the micromass culture system.
Conclusion: The study demonstrates that the C/S/GP hydrogel is potentially applicable as an injectable scaffold for cartilage tissue regeneration from hADSCs.
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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