| Research Title |
Biological factors affect the distribution of Burkholderia pseudomallei in soils |
| Date of Distribution |
20 September 2013 |
| Conference |
| Title of the Conference |
7 th WMC 2013 WORD MELIOIDOSIS CONGRESS 2013 |
| Organiser |
Mahidol Unversity, Wellcome Trust-Mahidol Unversity-Oxford Tropical Medicine Research programme |
| Conference Place |
Royal Orchid Sheraton Hotel & Towers |
| Province/State |
กรุงเทพมหานคร |
| Conference Date |
18 September 2013 |
| To |
20 September 2013 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
166 |
| Editors/edition/publisher |
|
| Abstract |
Burkholderia pseudomallei is a saprophytic bacteria resides in soil and stagnant water in endemic areas. B. pseudomallei is unevenly distributed in soil where the effective control methods for this pathogen is still appear to be limited. Studies on biological factors of soil, of which is the diversity of microbe in soil are important to understand the microbial community that might contribute to the survival of B. pseudomallei. The metagenomics strategy was used to investigate the population of microbes in soil in the presence and absence of B. pseudomallei. Soil physicochemical properties of the same site were also investigated. DNA extracted from 6 B. pseudomallei positive soil samples where number of DNA copies was found to be higher than colony number obtained from the direct culture method and 3 negative soil sites were selected. The DNA samples were amplified by PCR using universal primers for 16S rRNA gene that encompass bacteria in soil followed by 454 pyrosequencing to study soil bacterial community. The data were analyzed by RDP's Pyrosequencing Pipeline program. Six soil samples, when analyzed by 454 pyrosequencing indicated more acidobacteria group than the negative soil samples. On the other hand, soil sample without B. pseudomallei contained more Bacilli bacteria than soils sample with B. pseudomallei. The presence of acidobacteria group in positive soil is correlated with the acidic environment found in the presence of the bacteria. Further investigation is required to explore the contribution of them with B. pseudomallei in the same soil community. Organisms found in negative soil sites may produce antimicrobial substances or some inhibitors that influence the microbial ecology. These information will help us develop a tool to control or reduce the pathogenic organism in soil, which will decrease the possible infection of B. pseudomallei.
Keywords: Burkholderia pseudomallei, soil, Metagenomics, Pyrosequencing
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| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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