| Title of Article |
In Vitro Propagation and Cryopreservation of Aerides odorata Lour. (Orchidaceae) |
| Date of Acceptance |
28 August 2013 |
| Journal |
| Title of Journal |
Pakistan Journal of Biological Sciences |
| Standard |
|
| Institute of Journal |
Pakistan Journal of Biological Sciences |
| ISBN/ISSN |
1028-8880 |
| Volume |
1 |
| Issue |
1 |
| Month |
สิงหาคม |
| Year of Publication |
2013 |
| Page |
1-11 |
| Abstract |
An efficient method for in vitro propagation and cryopreservation of Aerides odorata was established. Leaf segments were cultured on New Dogashima (ND) mediums supplemented with various concentrations of Benzyladenine (BA) (0-5 mg L-1) combined with Naphthaleneacetic Acid (NAA) (0-2 mg L-1). The optimal treatment for inducing Protocorm-like Bodies (PLBs) from leaf segments was obtained from the combination of 1 or 3 mg L-1 BA and 0.5 or 1 mg L-1 NAA; whereas, the addition of BA or NAA alone induced shoot and/or root initiation rather than PLB or callus formation. Shoots rapidly developed on ND mediums containing 5 mg L-1 BA. Cryopreservation of leaf segment-derived PLBs was successful using the encapsulation-dehydration method. The maximum survival percentage of Cryopreserved (Cryp) PLBs was achieved by encapsulating PLBs with 2% Na-alginate combined with 2 M glycerol and 0.4 M sucrose. The encapsulated PLBs were then precultured in 0.75 M sucrose for 24 h and dehydrated for 6 h before plunging into liquid nitrogen. Genetic stability of Cryp PLBs after regrowth was assessed by flow cytometry. The findings showed no different patterns of ploidy levels and morphology between Cryp and non-cryopreserved (Ncryp) control plantlets. |
| Keyword |
micropropagation, cryopreservation,Aerides odorata Lour. , encapsulation-dehydration |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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