| Research Title |
The profile of hepatic cytochrome P450 activities in high fat and high fructose fed mice |
| Date of Distribution |
15 November 2013 |
| Conference |
| Title of the Conference |
6th Asian Association of Schools of Pharmacy (AASP) Conference 2013 |
| Organiser |
Asian Association of Schools of Pharmacy (AASP) |
| Conference Place |
Shaw Foundation House, National University of Singapore |
| Province/State |
Singapore |
| Conference Date |
26 November 2013 |
| To |
26 November 2013 |
| Proceeding Paper |
| Volume |
2013 |
| Issue |
1 |
| Page |
141-142 |
| Editors/edition/publisher |
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| Abstract |
Introduction
Cytochrome P450 (CYP450) is a superfamily of drug metabolized enzymes majority located in the liver. CYP450 plays an important role in catalyzing the oxidative biotransformation of endogenous compounds and xenobiotic substances such as drugs and environmental chemicals. Consuming of high fat and high fructose is one factor implicated to metabolic conditions and may cause hepatic pathology such as non-alcoholic fatty liver disease. Therefore, the present study aims to investigate the hepatic CYP450 activity of high fat- and high fructose-fed mice.
Experimental
Male ICR mice (n=5) were intragastrically administrated with 65% hydrogenated soil bean oil 1 mL/day and free access to 20% fructose in water daily for 8 weeks. The assessment of CYP450 enzymes activities was performed using responsive-catalytic reactions including O-dealkylation of ethoxyresorufin (EROD) and of methoxyresorufin (MROD) for CYP1A1 and CYP1A2, respectively, with O-dealkylation of benzyloxyresorufin (BROD) and pentoxyresorufin (PROD) for CYP2B9/10.
Results & Discussion
In high fat- and high fructose-fed mice, the level of CYP1A1 and CYP1A2 enzyme activity determined by EROD and MROD, respectively, was not significantly changed. The levels of CYP2B9/10 enzyme activity from PROD was significantly lowered from the second week of high fat- and high fructose feeding, whereas that determined by BROD was noted at the eight week of treatment. These observations supported that continuous consuming diet containing high fat- and high fructose could modify the hepatic CYP450 activities.
Conclusion
Modification of the enzymatic activities of hepatic CYP2B9/10 was significantly related to the duration of high fat- and high fructose-intake of mice. The change of CYP450 activity may possibly affect drug or xenobiotic metabolism resulted in ineffective treatment or increasing risk of toxicity. These observations suggested that the high-fat high-fructose-fed mice might be a useful animal model to predict study regulation of CYP450 activity related drug metabolism/drug interaction in implication of metabolic condition.
Keywords: Cytochrome P450 activities, High fat and high fructose diet
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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