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Publication
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| Research Title |
concurrent parasitic infection in endemic area of opisthorchiasis |
| Date of Distribution |
14 March 2011 |
| Conference |
| Title of the Conference |
NRCT-DFG International Wrokshop |
| Organiser |
Faculty of Medicine, KhonKaen university |
| Conference Place |
Faculty of Medicine, KhonKaen university |
| Province/State |
Khon Kaen |
| Conference Date |
14 March 2011 |
| To |
14 March 2011 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
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| Abstract |
It is known that northeast Thailand particulary KhonKaen province is the world hotspot of opisthorchiasis and cholangiocarcinoma. Among sample population of 1,280 in Chonnabot and Munchakiri districts, KhonKaen province, opisthorchiasis is the leading parasitic disease with the prevalence of 39.14%. The second common parasitic disease is strongyloidiasis with the prevalence of 8.9%, follow by infection with minute intestinal flukes, Taenia spp., Echinostoma spp., Hookworm (prevalence of 1.5-3.2%). In case of strongyloidiasis when diagnosed by agar plate culture technique (APCT), the prevalence became double (16.48%). Since strongyloidiasis is fatal from complicated disease especially in immunocompromised individuals or patients receiving long-term corticosteroid therapy. Accurate and efficient diagnosis is therefore essential for surveillance and effective treatment. At present, parasitological diagnoses of strongyloidiasis are problematic and often have low sensitivity and specificity due to low concentration of larvae in stool. Here we used Indirect enzyme-linked immunosorbent assay method (ELISA) to detect Strongyloides- specific IgG in serum and urine specimens. Receiver operating characteristic analysis was used to obtain a cutoff point for serodiagnosis of strongyloidiasis. Within the sub sample of 51 subjects with matched fecal, urine and serum specimens, the seroprevalence determined by urine and serum ELISA was 78.4% and 68.8%, respectively. By APCT the prevalence was 80.4%. Comparable prevalence of strongyloidiasis when determined by urine ELISA and that by parasitological methods suggested that the urine ELISA is more sensitive than serum ELISA. Particularly, within Strongyloides-negative subjects (n=9), urine ELISA is highly sensitive as more positive cases were detected (7 of 9, 77.8%) than that by the serum ELISA (3 of 9 cases, 33.3%). In conclusion, the established protocol for urine ELISA in diagnosis of strongyloidiasis showed similar sensitivity to serum ELISA and is also comparable to the standard APCT. In particular, the urine IgG ELISA is more sensitive in faecal negative cases. Since collection of urine is non invasive, the urine ELISA has potential applicability for diagnosis as well as mass screening of strongyloidiasis prior to a standard confirmatory test. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย