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Publication
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| Research Title |
High glucose condition promotes the aggressive phenotypes of cholangiocarcinoma cells via STAT3 activation |
| Date of Distribution |
11 June 2015 |
| Conference |
| Title of the Conference |
40th Annual Conference of the Malaysian Society of Biochemistry and Molecular Biology |
| Organiser |
The Malaysian Society of Biochemistry and Molecular Biology |
| Conference Place |
Putrajay Mariotte Hotel |
| Province/State |
Putrajaya, Malaysia |
| Conference Date |
10 June 2015 |
| To |
11 June 2015 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
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| Abstract |
The highest global incidence of cholangiocarcinoma (CCA) and national prevalence of Diabetes Mellitus (DM) are geographically overlapped in the Northeast of Thailand. There is, however, no report on the association of these two diseases in this area. Thus, thisThis study aimed to clarify whetherstudy the effect of high glucose condition in DM can promoteon the progression of CCA cell lines. CCA cell lines; KKU-M055, KKU-M213 and KKU-M214, were cultured in normal glucose (NG; 5.6 mM) and high glucose (HG; 25 mM) conditions for at least 5 passages and then subjected to the experiments. CCA cells in HG exerted more aggressive phenotypes than those in NG, including i.e., increased increasing of cell proliferation, adhesion, migration and invasion. The s Screening of the association signaling pathways using phospho-kinases array suggested STAT3 as a candidatepathway may molecule responsible for the higher aggression of cells in HG. The immunoblot also revealedconfirmed the increased phosphorylation of STAT3 (Y705 and S727) in HG compared with NG group. The expressions of STAT3’s downstream targets related to the aggressive phenotypes; namely cyclin D1, vimentin and matrix metalloproteinase 2, was were consistently up-regulated in HG group. The nuclear localization of STAT3 and p-STAT3 (S727) in CCA tissues from patients with high preoperative fasting blood glucose (≥126 mg/dL) was significantly higher than CCA tissuesthose from whom with normal lower blood glucose (<126 mg/dL ). Altogether, this study is the first to demonstrate the effect of HG on the progression of CCA. Then t The high blood glucose in CCA patients may be another a factor needed to be considered for the effective treatment of CCA. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย