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Publication
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| Research Title |
Role of RelA and SpoT in Ceftazidime Tolerance and Biofilm Formation of Burkholderia pseudomallei during Nutritional Depletion |
| Date of Distribution |
8 August 2016 |
| Conference |
| Title of the Conference |
8th World Melioidosis Congress 2016 |
| Organiser |
University of Florida |
| Conference Place |
Radisson Blu Cebu |
| Province/State |
Cebu, Philippines |
| Conference Date |
7 August 2016 |
| To |
10 August 2016 |
| Proceeding Paper |
| Volume |
8 |
| Issue |
1 |
| Page |
13 |
| Editors/edition/publisher |
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| Abstract |
Burkholderia pseudomallei, which is the causative agent of melioidosis, is intrinsically resistant to many antibiotics. The stringent response is a global bacterial adaptation process in response to nutritional limitation and is mediated by the alarmone (p)ppGpp, which is produced by RelA/SpoT homologue family (RSHs). In order to test whether the stringent response is involved in ceftazidime (CAZ) tolerance and biofilm formation, B. pseudomallei strain K96243 and the isogenic relA and relA spoT mutants were grown in rich and nutrient-limited media. The effect of nutrient-limited and enriched conditions on drug susceptibility and biofilm formation of B. pseudomallei were determined by broth microdilution method and a modified microtiter-plate test, respectively. The results showed that the minimal bactericidal concentrations (MBC) of CAZ against B. pseudomallei wild type and the isogenic relA and relA spoT mutants in nutrient-limited conditions were up to 64-times higher than those in rich culture conditions. The biofilm formation by all bacterial isolates tested was significantly higher under nutrient-limiting conditions than under nutrient-rich conditions (p < 0.001). Moreover, under nutrient-limiting conditions the relA spoT mutant produced 1.4 times less biofilm than its wild type and 1.2 times less biofilm than relA mutant. These data provides new information on the roles of RSHs of B. pseudomallei in antibiotic tolerance and biofilm-forming ability during nutrient-limited condition. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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