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Publication
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| Research Title |
Effect of Sericin Supplementation on Motility and Plasma Membrane Integrity of Frozen-Thawed Dairy Cattle Bull Semen |
| Date of Distribution |
23 August 2016 |
| Conference |
| Title of the Conference |
The 17th AAAP (Asian-Australasian Association of Animal Production Societies) Animal Science Congress |
| Organiser |
Kyushu Sangyo University |
| Conference Place |
Fukuoka, Kyusyu Area |
| Province/State |
Japan |
| Conference Date |
22 August 2016 |
| To |
25 August 2016 |
| Proceeding Paper |
| Volume |
2016 |
| Issue |
17 |
| Page |
355-356 |
| Editors/edition/publisher |
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| Abstract |
Semen cryopreservation decrease posted thawed sperm quality and affect to fertility. To improve the quality, antioxidant is considered as one of important factor should be supplemented in an extender. The present study was designed to improve semen quality by determine the effect of sericin supplementation (antioxidants) on sperm motility and plasma membrane integrity of frozen-thawed semen in dairy cattle. Fifteen semen samples from three bulls were collected by electroejeculation technique. The ejaculates were frozen in egg yolk-Tris-glycerol extender supplemented with different concentrations of sericin (0, 0.025, 0.05, and 0.1%). The semen quality including motility and plasma membrane integrity was assessed after freezing by phase contrast microscope and hypo-osmotic swelling test (HOST), respectively. Post-thawed semen evaluations showed that extenders supplemented with 0.025 and 0.05% increased sperm motility (P<0.01) meanwhile sperm motility was decreased in 0.1% but did not differ to control. The plasma membrane integrity was higher in group supplemented with 0.025% sericin compared with 0.05% (P<0.01). In conclusion, extender supplemented with 0.025% sericin improves sperm motility and plasma membrane integrity of frozen-thawed semen in dairy cattle. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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