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Publication
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| Title of Article |
Induction of regulatory T cells by Opisthorchis viverrini |
| Date of Acceptance |
19 August 2016 |
| Journal |
| Title of Journal |
parasite immunology |
| Standard |
ISI |
| Institute of Journal |
John Wiley & Sons Ltd |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2016 |
| Page |
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| Abstract |
Opisthorchis viverrini, causes public health problems in Southeast Asia. Recently, TGF-β and IL-10 have been reported to increase in O. viverrini infected hamsters but the sources of these cytokines are still unknown. In this study, the CD4+ T cells in infected hamsters were investigated. It was demonstrated that IL-4+ CD4+ T cells were significantly increased in hamster spleens and mesenteric lymph nodes (MLNs) during chronic infection. Interestingly, IL-10+ CD4+ T cells were also discovered at a significant level while Treg (T regulatory)-like TGF-β+ CD4+ T cells were in MLNs of infected hamsters. Moreover, the CD4+ CD25+ Foxp3+ Treg cell response was significantly found both in spleens and MLNs in infected hamsters. The findings were then confirmed by development of T cell clones against crude somatic antigens (CSAg) in immunized BALB/c mice. Five clones named TCC21, TCC23, TCC35, TCC41 and TCC108 were established. The TCC21 was found to be the TGF-β+ CD4+ while TCC35, TCC41 and TCC108 were IL-4+ CD4+ and TCC23 was IFN-γ+ CD4+ . This TGF-β+ CD4+ T clone showed an inhibitory function in vitro in mononuclear cell proliferation via TGF-β mediated mechanisms. This study indicated that O. viverrini infected hamsters could induce TGF-β+ CD4+ Treg-like cells. The CSAg specific Tregs secreted high TGF-β, and limited immune cell proliferation. |
| Keyword |
Opisthorchis viverrini, regulatory T cells, hamster, BALB/c mice |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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