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Publication
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| Research Title |
Studying the antimicrobial efficacy of Crocodylus siamensis hemoglobin hydrolysate (HH) |
| Date of Distribution |
21 March 2016 |
| Conference |
| Title of the Conference |
The microbiology society annual conference 2016 |
| Organiser |
The microbiology society |
| Conference Place |
Liverpool, United kingdom |
| Province/State |
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| Conference Date |
21 March 2016 |
| To |
24 March 2016 |
| Proceeding Paper |
| Volume |
2016 |
| Issue |
1 |
| Page |
1 |
| Editors/edition/publisher |
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| Abstract |
Antimicrobial proteins are one group of antimicrobial agents that are phylogenetically ancient components of innate host defense mechanisms and are expressed by immune and non-immune cells of both invertebrates and vertebrates. The biological activity of these agents demonstrates potency against a broad spectrum of microorganisms. This study aims to investigate antibacterial activity of hemoglobin hydrolysate (HH) from Crocodylus siamensis blood with differently peptic hydrolyzed at 2,4,6 and 8 hours. Antibacterial activity of hemoglobin hydrolysate was investigated by Minimal inhibition assay (MIC) and Live and dead staining assay using of both gram positive (S.aureus) and gram negative bacteria (E.coli, K.pneumoniae and P.aeruginosa), and β-galactosidase activity using E.coli 50, E.coli 175, E.coli 176, E.coli 190 and E.coli 191. The results showed that HH 2,4,6 and 8 hours exhibited antibacterial activity at the concentrations of 20 mg/ml (MIC value) against E.coli and S.aureus. Furthermore, there are exhibited antibacterial activity at the concentrations of 10 mg/ml (MIC value) against K.pneumoniae and P.aeruginosa. In live and dead staining assay found that HH 2,4,6 and 8 hours at the concentrations of 20 and 10 mg/ml can kill both of gram positive and gram negative bacteria; What is more, HH 8 hours has the highest activity when comparing with other sample. From Β-galactosidase assay found that when treated E.coli strains with HH 2,4,6 and 8 hours at the concentrations of 20 mg/ml can reduce ftNA expression and increase Bfd expression by observing miller units values. Therefore, this study shows that HH from Crocodylus siamensis blood might be having potential antimicrobial activity via iron regulation uptake. Further studies on bioactivities of HH are needed. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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