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Publication
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| Research Title |
The protective effect of white blood cell extract from Crocodylus siamensis blood on Propionibacterium acnes-induced inflammation in RAW 264.7 macrophages |
| Date of Distribution |
11 July 2017 |
| Conference |
| Title of the Conference |
The 5th Asia Pacific Protein Association and The 12th International Symposium of the Protein Society of Thailand Joint Conference |
| Organiser |
Protein Society of Thailand |
| Conference Place |
The Tide Resort, บางแสน |
| Province/State |
ชลบุรี |
| Conference Date |
11 July 2017 |
| To |
14 July 2017 |
| Proceeding Paper |
| Volume |
2017 |
| Issue |
1 |
| Page |
144 |
| Editors/edition/publisher |
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| Abstract |
White blood cells (WBC) are an important part of the immune system involved in protecting the body against both infectious disease and foreign invaders, which might be associated to inflammation. Thus, the aim of this work was to study the anti-inflammatory activity of Crocodylus siamensis WBC extract by assessment of nitric oxide (NO) production in RAW 264.7 macrophages stimulated with Gram-positive Propionibacterium acnes. Prior to evaluating the anti-inflammatory properties of WBC extract, the in vitro cytotoxicity against RAW 264.7 cells was investigated using the MTT assay. The results showed that WBC extract in a concentration range of 25-100 µg/ml exhibited no cytotoxic effects toward RAW 264.7 cells. Consequently, similar doses were chosen for the next experiments. For the inflammation assay, heat-killed P. acnes was prepared using two individual methods involving either heating at 95 °C for 10 min or autoclaving. Both P. acnes preparations were employed to stimulate the inflammatory response in RAW 264.7 cells with or without co-incubation with WBC extract at different concentrations (25, 50 and 100 µg/ml) for 24 h. The obtained results suggest that the bacteria could induce NO production in macrophages under all tested conditions, indicating an inflammatory state was induced within the cells. However, a significant reduction of NO production was effected by the WBC extract at all tested concentrations. Therefore, the results demonstrate that C. siamensis WBC extract exhibits anti-inflammatory activity, presumably by modulating NO production in macrophages that were stimulated by P. acnes. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย