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Publication
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| Research Title |
Gold nanoparticle-graphene-chitosan modified disposable electrode for detection of prohormone Dehydroepiandrosterone sulfate |
| Date of Distribution |
21 July 2017 |
| Conference |
| Title of the Conference |
The 7th National and International Graduate Study Conference (IGSC 2017) |
| Organiser |
Graduate School, Silpakorn University |
| Conference Place |
Princess Maha Chakri Sirindhon Anthropology Centre |
| Province/State |
Bangkok |
| Conference Date |
20 July 2017 |
| To |
21 July 2017 |
| Proceeding Paper |
| Volume |
2017 |
| Issue |
1 |
| Page |
417-425 |
| Editors/edition/publisher |
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| Abstract |
Dehydroepiandrosterone sulfate (DHEAs) is the most abundant circulating prohormone in human. DHEAs has been known as a biomarker of aging because DHEAs levels in human decline markedly with age. There are many techniques for determination of DHEAs including HPLC, GC-MS, LC-MS and ELISA. However, these techniques are not available for application in point of care testing. The objective of this study is to prepare electrochemical immunosensor based on gold nanoparticle-graphene-chitosan (AuNPs-GP-CS) for DHEAs detection because this technique has high sensitivity and specificity, simple to use, cost effective, using small samples volume and portable device. AuNPs-GP-CS nanocomposite was synthesized and characterized by UV-vis spectroscopy and transmission electron microscope. Amperometric detection was performed at a potential of -0.2 V using 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 as substrate solution. The experimental conditions were optimized, including the concentration of anti-DHEAs antibody, dilution ratio of DHEAs-HRP conjugate and incubation time. Under optimal conditions, this immunosensor showed a linear range from 0.001-10 ug mL-1 with a detection limit of 0.089 ug mL-1. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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