| Abstract |
The objectives of this research, were investigated the spore density and species diversity of arbuscular mycorrhizal fungal (AMF) in rhizosphere soil from 10 different sites of agricultural eucalyptus field in the northeast of Thailand, including Khon Kaen, Maha Sarakham, Udon Thani and Nongbualamphu provinces. AMF spores were extracted by the wet sieving and decanting, and sucrose gradient centrifugation methods. Root colonization of AMF and spore counting were assessed under compound microscope. The roots of eucalyptus were colonized by AMF that could be found many of AMF structure such as hyphae, vesicles, and arbuscules. The percentage of root colonization of AMF ranged from 0.67 to 51.38% (average 39.11) and the spore density in 1g of dry soil was ranging from 46.00 to 165.67 spore (average 83.00). In addition, the identification of AMF distributed in rhizosphere soil from eucalyptus tree planted in each sites was carried out. The AMF were morphologically identified on the basis of spore characteristic followed by the manual for the identification of VA mycorrhizal fungi (Schenck and Perez, 1988) and International Culture Collection of VA Mycorrhizal Fungi (INVAM). The result revealed that 8 morphospecies of AMF namely, Acaulospora foveata, A. sporocarpia, Gigaspora albida, Gi. decipiens, Glomus albidum, Gl. invermaium, Gl. magnicaule, and Glomus sp. could be identified. |
Publication
Journal Publication
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