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Publication
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| Title of Article |
Immunohistochemical localization of cannabinoid receptor 1 (CB1) in the submandibular gland of mice under normal conditions and when stimulated by isoproterenol or carbachol |
| Date of Acceptance |
10 May 2017 |
| Journal |
| Title of Journal |
Archives of Oral Biology |
| Standard |
SCOPUS |
| Institute of Journal |
ELSEVIER |
| ISBN/ISSN |
0003-9969 |
| Volume |
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| Issue |
81 |
| Month |
September |
| Year of Publication |
2017 |
| Page |
160-166 |
| Abstract |
Objective:We wished to investigate the subcellular localization of CB1, a receptor for the endocannabinoids in
mouse submandibular glands (SMGs) under normal conditions and when stimulated by adrenergic or cholinergic
agonists.
Materials and methods:SMGs of both male and female adult mice were utilized for immunoblotting and immunolight and –electron microscopic analyses. Isoproterenol and carbachol were used as adrenergic and cholinergic
stimulants, respectively. SMGs were examined at 15, 30, 60 and 120 min after intraperitoneal injection of these
agents.
Results:Selective localization of intense immunoreactivity for CB1 in the granular convoluted ductal cells was
confirmed by immunoblotting and the antigen absorption test. In SMGs of control male mice, CB1-immunoreactivity was evident on the basolateral plasma membranes, including the basal infoldings, but was
absent on the apical membranes in the ductal cells. Localization and intensity of CB1-immunoreactivity were
essentially the same in SMGs of female mice. The immunoreactivity was transiently localized in the apical
plasmalemma of some acinar and granular ductal cells of male SMGs shortly after stimulation by isoproterenol,
but not by carbachol.
Conclusion:The presentfinding suggests that CB1 functions primarily in the basolateral membranes of the
granular convoluted ductal cells of SMGs under normal conditions, and that the CB1 can function additionally in
the apical membrane of acinar and granular ductal cells for modulation of the saliva secretory condition via
adrenoceptors. |
| Keyword |
CB1, Isoproterenol, Acinar cell, Granular convoluted duct cell, Mouse submandibular gland |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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