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Publication
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| Research Title |
Preliminary study of RNA polymerase β subunit (rpoB) gene mutations in vancomycin-susceptible and non-susceptible Staphylococcus aureus isolates from a Thai university hospital |
| Date of Distribution |
14 September 2017 |
| Conference |
| Title of the Conference |
11th International Symposium on Antimicrobial Agents and Resistance (ISAAR 2017) 3rd International Interscience Conference on Infection and Chemotherapy (ICIC 2017) |
| Organiser |
Korean Federation of Science and Technology Societies (KOFST) grant funded by the Korean Government. |
| Conference Place |
BEXCO, Busan, Korea |
| Province/State |
Busan |
| Conference Date |
14 September 2017 |
| To |
16 September 2017 |
| Proceeding Paper |
| Volume |
2017 |
| Issue |
50 |
| Page |
S1-S176 |
| Editors/edition/publisher |
J.-M. Rolain, (Marseille, France)/Elsevier B.V. and the International Society of Chemotherapy. |
| Abstract |
Preliminary study of RNA polymerase β subunit (rpoB) gene mutations in vancomycin-susceptible and non-susceptible Staphylococcus aureus isolates from a Thai university hospital
S. Wongthong1,3*, P. Tippayawat2,3, A. Chanawong2,3, Tavichakorntrakool R2,3, Wilailuckana C2,3 and A. Lulitanond2,3
1Graduate School, 2Centre for Research and Development of Medical Diagnostic Laboratories, 3Faculty of Associated Medical Sciences, Khon Kaen University, Thailand
Background: The global uses of vancomycin to treat methicillin-resistant Staphylococcus aureus (MRSA) lead to the development of vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA). Some VISA and hVISA isolates were reported to have rpoB mutation, which may play a major role in the development of vancomycin resistance in S. aureus. This gene encodes for a β-subunit of RNA polymerase, which is active in catalysis reaction. Some mutations in rpoB conferred transcriptional activity of the whole RNA polymerase. The most common mutation of rpoB in VISA was a substitution of H481Y. Therefore, we aimed to examine the rpoB mutations in MRSA isolates from a Thai university hospital.
Methods: Eleven MRSA isolates were classified for their vancomycin resistant phenotypes by using population analysis profile with area under the curve (PAP-AUC) method. The nucleotide sequences of rpoB from all isolates were determined and compared with that of the wild type isolate.
Results: The 10 MRSA isolates included 3 VSSA, 2 hVISA and 5 VISA with their PAP-AUC ranges of 0.85-0.86, 0.92-1.05 and 1.46-1.91 respectively. All isolates contained various rpoB mutations. One isolates of VSSA, 2 hVISA and 2 VISA had amino acid substitution at H481N and/or S529L, similar to those previously reported in the MRSA isolates from several countries including Thailand, In addition, other nucleotide substitutions were found the amino acid positions 348 to 667 of RpoB. Only one of VISA isolate has cytosine deletion at 843 of nucleotide sequence therefore an amino acid at position 640 was change from an alanine to be glycine (A640G). Moreover, the next amino acid at position 641 to 649 were also changed. At position 649, amino acid was changed from leucine to be stop codon.
Conclusion: Some mutations in rpoB gene may be related to vancomycin resistance in S. aureus and should be further studied.
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย