| Abstract |
A simultaneous saccharification and fermentation (SSF) process
was applied for thermophilic bio-hydrogen production from lime-pretreated
oil palm trunk (OPT) by Thermoanaerobacterium thermosaccharolyticum
KKU19. The SSF hydrogen fermentation conditions were optimized to
maximize hydrogen yield (HY). Based on Plackett-Burman design, substrate
loading and initial pH had significant effects on HY. The substrate
loading and initial pH were further optimized using response surface
methodology with a central composite design. The optimum conditions were
a substrate loading, enzyme loading, inoculum concentration, initial pH
and temperature of 4.6%, 10 filter paper unit (FPU)/g-OPT, 10% (v/v), 6.3
and 50 oC, respectively, which yielded the highest HY of 60.22 mL H2/g-
OPT. Structural analysis showed that lime pretreatment and SSF decreased
the crystallinity of OPT. Methane production was carried out following
the hydrogen production to improve the energy yield from OPT. The results
showed that methane production increased total energy yield from 0.65 to
11.79 kJ/g OPT under the optimal conditions. |
Publication
Journal Publication
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