| Abstract |
Little is known about the effects of Cholesterol-Loaded Cyclodextrin (CLC) on post-thaw semen quality in
chicken. The aim of the present study is to investigate the efficacy of CLC levels (0, 1, 2 and 3 mg/mL
Schramm diluent) on post-thawed semen quality and fertility in two breeds of chicken Pradu Hang Dum
(native chicken) and Rhode Island Red. Semen samples of each breed were pooled, divided into 4 aliquots
and diluted with Schramm diluents, cooled to 5 C when DMF was added (6% of final volume). Semen
straws were subjected to cryopreservation using the liquid nitrogen vapor method. Post-thawed sperm
motility, viability, acrosome integrity, mitochondrial function, and the Malondialdehyde (MDA) level
were determined. The fertility of frozen semen was tested by inseminating laying hens. Post-thaw
motility between Pradu Hang Dum and Rhode Island Red was no different; but Rhode Island Red had
a higher semen viability and live cell intact acrosomes than Pradu Hang Dum (P < 0.05). The percentage
of high functioning mitochondria in the Pradu Hang Dum was higher than the Rhode Island Red. CLC at 2
and 3 mg/mL supplementationwas associated with improved viability of frozen semen; that is, acrosome
integrity and mitochondrial function (P < 0.01), albeit having no effect on MDA levels. The sperm with
1 mg/mL CLC yielded a significantly better fertility (P < 0.01). CLC (1 mg/mL) improved the quality of
frozen rooster semen. There was no interaction among breeds and CLC on post-thaw semen quality and
fertility |
Publication
Journal Publication
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