| Abstract |
Methomyl, an insecticide belonging to the carbamate types, is an extremely toxic compound that is widely used in agriculture. It exhibits negative effects on human health upon a long-term exposure. Thus, it is necessary to find natural substances that can detoxify this insecticide. Herbs are widely used for therapeutic treatment in local wisdom of Thai people, especially Yanang (Tiliacora triandra) which possesses an effective detoxification property. Therefore, this study aimed to investigate the protection property of Yanang water extract on methomyl induced cytotoxicity on murine macrophage cell lines (RAW 264.7 cells). The hemolytic activity assay was used for primary screening of the non-toxic concentration rage of Yanang extract. The obtained result confirmed that Yanang extract at a concentration of 6.25, 12.5 and 25 μg/ml exerted no toxicity towards human red blood cells. Consequently, the dosages of 5, 10 and 20 μg/ml of Yanang extract were evaluated for the protective effects agianst methomyl induced cytotoxicity in RAW 264.7 cells. The results demonstrated that Yanang extract exhibited no toxicity towards RAW 264.7 cells and could promote cell viability of methomyl induced RAW 264.7 cells. In conclusion, the current study suggested that Yanang might contain particular agents with a potential for methomyl detoxification. |
Publication
Journal Publication
ไทย