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Publication
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| Research Title |
CHARACTERIZATION OF MODIFIED CROCODILE (Crocodylus siamensis) HEMOGLOBIN USING BIS(3,5-DIBROMOSALICYL) FUMARATE |
| Date of Distribution |
20 June 2018 |
| Conference |
| Title of the Conference |
The 6th International Conference on Biochemistry and Molecular Biology |
| Organiser |
Biovhemistry and Molecular Biology Section of the Science Society of Thailand under the Patronage of His Majesty the King and Department of Biochemistry, Faculty of Science, Chulalongkorn University |
| Conference Place |
Rayong Resort |
| Province/State |
Rayong, Thailand |
| Conference Date |
20 June 2018 |
| To |
22 June 2018 |
| Proceeding Paper |
| Volume |
2018 |
| Issue |
1 |
| Page |
68 |
| Editors/edition/publisher |
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| Abstract |
A variety of cross-linked cell-free hemoglobin (CL-Hb) compounds have been developed with the aim of creating novel hemoglobin-based oxygen carriers (HBOCs). In general, derived CL-Hb using different cross-linking agents exhibits a lower oxygen affinity than native hemoglobin. Therefore, crocodile (C. siamensis) hemoglobin (cHb) constitutes an interesting new approach in HBOC development, as it was reported to possess a higher oxygen affinity than human hemoglobin (hHb). In this study, we performed a modified cHb through cross-linking with bis-3,5-dibromosalicyl fumarate (DBBF) and aimed to investigate its basically physiological and biological functions as comparing with hHb. The DBBF-cHb plays a different characteristic of cross-linking, since there was the absence of ββ-dimer band on SDS-PAGE. It is important to note that DBBF-cHb exhibited a higher oxygen affinity than DBBF-hHb with P50 value of 20.1 mm Hg (n = 2.5). In conclusion, cHb exhibited oxygen binding characteristics that may benefit the development of a cHb-based oxygen carrier suitable for human use in the future. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย